Lycopene and Beta-carotene Metabolism in the Digestive Tract of Healthy Men

Summary

Consumption of foods containing carotenoids, as well as vitamin E, have been associated with lower risk of developing a number of chronic diseases. While the parent compounds have largely been assumed to exert protective antioxidant effects, more recent work has suggested that metabolites may be bioactive. Very little attention has been given to the metabolism of these compounds during the digestive process. Our primary aim is to conduct a postprandial feeding study in healthy men to determine the stability of carotenoids and vitamin E during digestion, and to identify the primary metabolites produced in various compartments of the upper gastrointestinal tract and blood during digestion. Targeted metabolites will be identified and quantitated using high-performance liquid chromatography-tandem mass spectrometry methods previously developed. In addition, a non-targeted metabolomics approach will be used to identify non-predicted metabolites in the samples. A better understanding of carotenoid and vitamin E stability and metabolism during digestion will provide greater insight into how these compounds may confer protection against chronic disease.

Conditions

• Digestion Chambers
• Stability and Metabolism of Carotenoids and Vitamin E

Interventions

OTHER

lycopene

A tomato oleoresin containing lycopene

OTHER

beta-carotene

13C beta-carotene

OTHER

control

emulsified liquid meal alone

Sponsors & Collaborators

• Aix Marseille Université

  collaborator OTHER

• Hôpital de la Conception

  collaborator UNKNOWN

• Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

  lead OTHER

Principal Investigators

• Patrick Borel, PhD · INRA/INSERM/Université Aix-Marseille

• Catherine Caris-Veyrat · INRA/Université d'Avignon

Study Design

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Model

PARALLEL

Eligibility

Min Age

18 Years

Max Age

50 Years

Sex

MALE

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2014-09-30

Primary Completion

2016-03-31

Completion

2016-03-31

Countries

• France

Study Locations