Diet Composition and Physical Inactivity on Insulin Sensitivity and β-cell Function

Summary

Physical inactivity results in reductions in glucose tolerance and less sensitivity to insulin. If this inactivity lasts long enough it can result in insulin resistance and type 2 diabetes. A high protein diet can reduce elevated glucose levels in individuals with type 2 diabetes. Thus the investigators are interested in establishing if during a period of inactivity if a diet modification can minimize the glucose changes normally observed with inactivity. The objective of this project is to determine if short-term high protein (HP) feeding protects against the changes in glucose levels normally observed with physical inactivity. The investigators will also examine measures of blood vessel function, blood lipid and blood pressure.

Twelve subjects will complete two 10 day study periods of reduced physical activity and will be studied before and after each of these study periods. For their testing subjects will have the following measurements: postprandial glucose responses to a mixed meal, 24 h free living blood pressure control during acute physical inactivity, blood lipids, changes in body composition, changes in circadian rhythm using skin temperature (ibutton), measurement of aerobic capacity (VO2 max), blood vessel responsiveness (flow mediated dilation -FMD) and changes in free living glucose levels (continuous glucose monitoring system (CGMS). Subjects will complete two conditions (high protein -HP vs normal protein - NP diets) in a randomized cross-over design. In the inactive phase subjects will reduce there steps to \<5,000 steps/d while consuming either a HP or NP diet. Completion of the study will take 8-10 weeks.

Conditions

• Physical Inactivity
• Dietary Protein

Interventions

BEHAVIORAL

normal protein diet

subjects will undergo 10 days of low physical activity while consuming a normal protein diet

BEHAVIORAL

high protein intake

subjects will undergo 10 days of low physical activity while consuming a high protein diet

Sponsors & Collaborators

• American Egg Board

  collaborator OTHER

• University of Missouri-Columbia

  lead OTHER

Principal Investigators

• jill kanaley, Phd · University of Missouri-Columbia

Study Design

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Model

CROSSOVER

Eligibility

Min Age

18 Years

Max Age

45 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2015-10-31

Primary Completion

2017-09-30

Completion

2017-09-30

Countries

• United States

Study Locations