Modified Breath Test to Determine Anabolic Sensitivity Across Physical Activity States

Summary

Developing tools to detect when our bodies are more resistant towards protein synthesis is valuable for identification of when someone may be at risk of losing body or muscle mass such as with aging or certain diseases. The current study aims to refine our previous breath test method to be more effective at measuring changes in how the body processes protein in different situations, such as resting, reducing physical activity, and doing resistance exercise. We hypothesize that using a lower amount of dietary amino acids in our breath test will be effective at detecting lower amounts of amino acids used after exercise, and a greater amount with step reduction compared to normal activity levels

Conditions

• Resistance Exercise
• Physical Inactivity
• Amino Acids
• Dietary Protein

Interventions

BEHAVIORAL

Habitual Activity

Participants will maintain habitual levels of physical activity (inclusive of structured physical activity).

BEHAVIORAL

Step-Reduction

Participants will be required to reduce their daily step-counts to \<2,000 steps/day. Further, they will be required to refrain from structured physical activity.

BEHAVIORAL

Resistance Exercise

Participants will undergo a 50-minute resistance exercise protocol, which includes multiple sets of different exercises using weights. Each set will consist of 10 repetitions at 75% of their 1 repetition maximum (1RM). The exercises include bench press superset with lat pulldowns, overhead press superset with seated cable rows, leg press, and leg extensions with 90s rest in between sets. Before the exercise protocol, there will be a standardized 10-minute warm-up that involves cycling, leg swings, arm circles, bodyweight squats, and bench push-ups

Sponsors & Collaborators

• University of Toronto

  lead OTHER

Principal Investigators

• Ines Kortebi, MSc · University of Toronto

• Hugo JW Fung, PhD (c) · University of Toronto

• Daniel R Moore, PhD · University of Toronto

Study Design

Allocation

RANDOMIZED

Purpose

OTHER

Masking

NONE

Model

CROSSOVER

Eligibility

Min Age

18 Years

Max Age

35 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2024-01-15

Primary Completion

2024-12-31

Completion

2025-04-30

Countries

• Canada

Study Locations