Adipose Tissue Pre and Post Bariatric Surgery

Summary

Published data suggest that inflammation and fibrosis of adipose tissue could be factors favoring the development of insulin resistance in obese individuals and that a decrease in the activity of the AMP-activity kinase protein (AMPK) could lead to these dysfunctions. However, very few data are available in humans.

There is also growing interest in persistent organic pollutants (POPs) as a cardiometabolic and type 2 diabetes (T2D) risk factor. There is some evidence to suggest that POPs directly contribute to lipid metabolism dysfunction and insulin resistance. Additionally, POPs are stocked in adipose tissue. The accumulation of POPs in adipose tissue therefore limits their bioavailability to other organs, thus reducing their systemic toxicity. It has been observed that a large amplitude weight loss leads to a significant increase in POPs in the blood.

The goal of this project is to identify adipose tissue factors/dysfunctions that contribute to insulin resistance and type 2 diabetes associated with obesity in humans and thus raise avenues for screening and treatment of these metabolic complications. More specifically, the objectives are:

* To study the relationship between AMPK, fibrosis and inflammation of adipose tissue and their role in the development of insulin resistance and T2D associated with obesity;
* To examine the relationship between POPs and the cardiometabolic profile.

Conditions

• Obesity

Interventions

PROCEDURE

Bariatric surgery

Sleeve gastrectomy

OTHER

Blood sampling

Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants

OTHER

Blood pressure

Systolic and diastolic blood pressure will be measured.

OTHER

Anthropometric measurements

Body weight, height and waist circumference will be measured.

OTHER

Body composition

Body composition will be evaluated by dual-energy X-ray absorptiometry.

OTHER

Resting metabolic rate

Resting metabolic rate will be measured by indirect calorimetry.

OTHER

Food intake

Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.

PROCEDURE

Adipose tissue biopsy

A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Sponsors & Collaborators

• Institut de Recherches Cliniques de Montreal

  lead OTHER

Principal Investigators

• Rémi Rabasa-Lhoret · Institut de recherches cliniques de Montréal

Eligibility

Min Age

18 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2012-04-25

Primary Completion

2022-07-31

Completion

2022-09-30

Countries

• Canada

Study Locations