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GCF miRNA-223 , -214 Levels in Smokers and Nonsmokers Periodontitis Patients

NCT06064799 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 42

Last updated 2023-10-03

No results posted yet for this study

Summary

Objective: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis.

Materials and Methods: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smoker with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.

Conditions

  • Periodontitis, Adult

Interventions

DIAGNOSTIC_TEST

miRNA-223 & -214 detection

miRNA-223 \& -214 detection in GCF samples by PCR kits

Sponsors & Collaborators

  • Fayoum University

    lead OTHER

Eligibility

Min Age
20 Years
Max Age
60 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2023-01-01
Primary Completion
2023-06-30
Completion
2023-06-30

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT06064799 on ClinicalTrials.gov