Effect of Periodontal Treatment on Visfatin, fetuin-a and Sirtuin 1 of Patients With Periodontitis and Type 2 Diabetes

Summary

The aim of this study is to determine the concentrations of Visfatin, Fetuin-A and Sirtuin 1 in the gingival crevicular fluid and clinical periodontal parameters in diabetic and systemically healthy individuals and to determine whether non-surgical periodontal treatment had any effect on these biomarkers and periodontal clinical parameters at the end of a 3-month follow-up period. The hypothesis of our study is that gingival crevicular fluid Visfatin, Fetuin-A and Sirtuin 1 concentrations will change with non-surgical periodontal treatment in type 2 diabetic and systemically healthy individuals and that this change will be associated with diabetes and clinical parameters.

Conditions

• Periodontal Diseases
• Type 2 Diabetes
• Periodontitis

Interventions

DIAGNOSTIC_TEST

GCF (gingival crevicular fluid) collection

Prior to GCF (gingival crevicular fluid) sampling, supragingival plaque was removed by sterile curets and, after air drying, the surfaces were isolated by cotton rolls. Gingival fluid collection strips were placed in sulcus for 30s. Care was taken not to avoid mechanical trauma and strips contaminated with blood or saliva were discarded. The absorbed GCF volume was estimated by a calibrated instrument. Then, the strips were sealed into sterile tubes before freezing at -80 °C. The readings were converted to an actual volume (μl) by reference to the standard curve.

PROCEDURE

Non-surgical periodontal treatment completed in 24 hours

After clinical periodontal parameters were recorded and GCF samples were obtained, non-surgical periodontal treatment was started under local anesthesia. All periodontal treatments were performed by a single investigator. Local infiltrative anesthesia was applied to the buccal and palatal/lingual areas of the maxilla and mandible of the patients. After anesthesia was achieved, scaler and ultrasonic tips of various thicknesses were used together to remove supragingival and subgingival hard attachments. Root surface smoothing was performed with region-specific periodontal curettes. The roughness of the surfaces was controlled using a periodontal probe. Polishing was performed. Oral hygiene education was given to each patient after treatment. Modified Bass technique was explained as a brushing technique. Interdental cleaning is explained. The patient was told not to use any chemical agent for plaque removal.

DIAGNOSTIC_TEST

HbA1c level determination in blood serum

Blood samples were taken from the patients and HbA1c concentration was measured in serum.

Sponsors & Collaborators

• Ondokuz Mayıs University

  lead OTHER

Principal Investigators

• Burcu OZKAN CETINKAYA, Prof. · Ondokuzmayis University, Faculty of Dentistry, Department of Periodontology, Samsun, Turkey.

• Aysun AYDEMIR INAM, Dr. · Ondokuzmayis University, Faculty of Dentistry, Department of Periodontology, Samsun, Turkey.

• Bahattin AVCI, Prof. · Ondokuzmayis University, Faculty of Medicine Department of Biochemistry, Samsun, Turkey.

Study Design

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

TRIPLE

Model

PARALLEL

Eligibility

Min Age

31 Years

Max Age

81 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2021-11-15

Primary Completion

2022-03-14

Completion

2022-07-15

Countries

• Turkey (Türkiye)

Study Locations