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High-Intensity Interval Training and Monocyte Epigenetic Adaptation After Detraining

NCT07771075 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 80

Last updated 2026-08-18

No results posted yet for this study

Summary

This randomized, work-matched training-detraining-rechallenge study evaluated whether repeated high-intensity interval training produces persistent functional and epigenetic adaptations in human monocytes. Eighty healthy physically inactive adults aged 18-30 years were randomly assigned in a 2:2:1 ratio to high-intensity interval training (HIIT), moderate-intensity continuous training (MCT), or a non-training control group. HIIT and MCT completed 6 weeks of supervised cycling training matched for external mechanical work, followed by 4 weeks without prescribed structured training. All groups subsequently completed a standardized 30-minute cycling rechallenge. The primary outcome was the between-group difference in change in ex-vivo LPS-stimulated TNF-alpha release from baseline to the post-detraining assessment. Secondary outcomes included IL-6 release, H3K18 lactylation, monocyte subset distribution, blood lactate exposure, chromatin accessibility, H3K18la CUT\&Tag occupancy, and RNA-seq transcriptional profiling.

Conditions

  • Physical Inactivity

Interventions

BEHAVIORAL

High-Intensity Interval Training

Supervised cycling HIIT performed three sessions per week for 6 weeks. Each session included a 5-minute warm-up at 30% MAP, four 4-minute intervals at 85% MAP separated by three 3-minute active recovery periods at 40% MAP, and a 5-minute cool-down at 30% MAP.

BEHAVIORAL

Moderate-Intensity Continuous Training

Supervised moderate-intensity continuous cycling performed three sessions per week for 6 weeks. Each session included a 5-minute warm-up at 30% MAP, 31 minutes and 16 seconds of continuous cycling at 55% MAP, and a 5-minute cool-down at 30% MAP. The protocol was designed to match the external mechanical work performed by the HIIT group.

Sponsors & Collaborators

  • Saif Rasheed Ghanim

    lead OTHER

Principal Investigators

  • Saif Rasheed Ghanim, PhD · University of Samarra, College of Physical Education and Sports Sciences

Study Design

Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Model
PARALLEL

Eligibility

Min Age
18 Years
Max Age
30 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2026-01-14
Primary Completion
2026-06-21
Completion
2026-07-02

Countries

  • Iraq

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07771075 on ClinicalTrials.gov