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Salivary TNF-α, FoxO-1, and SIRT-1 Levels in Periodontitis and Smoking

NCT07764549 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 80

Last updated 2026-08-14

No results posted yet for this study

Summary

This study examines whether smoking and gum disease (periodontitis) are linked to changes in three biological markers found in saliva: TNF-α, FoxO-1, and SIRT-1. These markers are involved in inflammation and the body's response to stress.

Eighty adults took part in the study, divided into four groups: healthy non-smokers, non-smokers with Stage III periodontitis (an advanced form of gum disease), healthy smokers, and smokers with Stage III periodontitis. Each participant had a dental examination and provided a saliva sample, which was analyzed in the laboratory to measure the levels of these three markers.

The goal of the study is to better understand how smoking and gum disease together affect the body's inflammatory and stress-response pathways, which may help guide future approaches to prevention and treatment.

Conditions

  • Periodontitis (Stage 3)
  • Smoking ( Cigarette)

Interventions

DIAGNOSTIC_TEST

Salivary Biomarker Analysis (ELISA)

Unstimulated whole saliva samples were collected via passive drooling and analyzed for TNF-α, FoxO-1, and SIRT-1 concentrations using commercially available enzyme-linked immunosorbent assay (ELISA) kits (BT LAB, Korain Biotech, Shanghai, China), according to the manufacturer's instructions. Concentrations were adjusted according to individual salivary flow rate.

Sponsors & Collaborators

  • Zehra Hasgül

    lead OTHER

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2025-10-24
Primary Completion
2026-03-24
Completion
2026-05-24

Countries

  • Turkey (Türkiye)

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07764549 on ClinicalTrials.gov