Salivary Proteome in Response to Non-surgical Periodontal Treatment

Summary

This study aims to investigate salivary proteome changes in periodontitis patients before and after non-surgical treatment. Ten systemically healthy and non-smoker individuals with stage III, grade C periodontitis underwent non-surgical periodontal treatment. Saliva was collected at baseline, and one and six months post-treatment. Whole-mouth plaque and gingival index, probing depth, bleeding on probing and clinical attachment loss were measured. The saliva proteome was investigated by label-free quantitative proteomics. Normalized protein intensities were measured and protein changes were modeled over time with significant protein regulation considered at false discovery rate (FDR)\<0.05.

Conditions

• Periodontitis

Interventions

PROCEDURE

Conventional quadrant-wise scaling and root planing (SRP)

SRP was conducted at baseline, after saliva collection and periodontal assessment, starting with the upper right quadrant and continuing clockwise over four visits at weekly intervals. SRP was performed under local anesthesia by the same periodontist (B.A.) by use of ultrasonic instruments (Mini Piezon, EMS, Nyon, CH) and manual periodontal curettes (Gracey curets, scaler Hu-Friedy, Chicago, IL). All present teeth were instrumented until the root surface was visually and tactilely clean and smooth. Self-performed plaque control measures consisted of toothbrushing using the modified Bass technique with a medium toothbrush and a regular toothpaste with fluoride twice a day and interdental cleaning using dental floss and/or interdental brushes once a day.

Sponsors & Collaborators

• Aydin Adnan Menderes University

  lead OTHER

Principal Investigators

• Angelika Silbereisen · Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

• Kai Bao · Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

• Witold Wolski · Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

• Paolo Nanni · Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

• Laura Kunz · Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Switzerland

• Beral Afacan · Department of Periodontology, Faculty of Dentistry, Adnan Menderes University, Aydın, Turkey

• Gülnur Emingil · Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey

• Nagihan Bostanci · Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden

Eligibility

Min Age

32 Years

Max Age

43 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2018-07-01

Primary Completion

2019-06-30

Completion

2019-12-30

Countries

• Turkey (Türkiye)

Study Locations