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EVALUATION OF SALIVARY BİOMARKER LEVELS IN SMOKING AND NON-SMOKER INDIVIDUALS WITH DIFFERENT PERIODONTAL DISEASES

NCT06971770 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 80

Last updated 2025-05-14

No results posted yet for this study

Summary

This study aimed to compare various cytokine levels in saliva samples in order to understand the pathogenesis of chronic inflammatory diseases. In particular, HMGB1, sTREM-1 and TNF-α levels were examined between smokers and non-smokers with periodontitis and non-smokers with gingivitis and periodontally healthy individuals. 80 systemically healthy individuals were included in the study. These individuals were divided into four groups: 20 non-smoker periodontally healthy, 20 non-smoker with gingivitis, 20 non- smokers with periodontitis and 20 smokers with periodontitis. Periodontal clinical parameters of each individual were measured and saliva samples were taken. HMGB1, sTREM-1 and TNF-α levels in the saliva samples were determined by enzyme-linked immunoassay (ELISA) method. Statistical analyses were performed on the data to examine the differences in cytokine levels between the groups and the relationships of these cytokines with clinical parameters. The findings obtained may contribute to the research of potential biomarkers that can be used in the diagnosis of periodontal diseases and new ways of treatment processes.

Conditions

  • Periodontal Diseases

Interventions

DIAGNOSTIC_TEST

saliva obtaining

The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.

Sponsors & Collaborators

  • Izmir Katip Celebi University

    lead OTHER

Study Design

Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Model
PARALLEL

Eligibility

Min Age
18 Years
Max Age
70 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2024-04-01
Primary Completion
2024-11-01
Completion
2025-02-01

Countries

  • Turkey (Türkiye)

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT06971770 on ClinicalTrials.gov