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Phenotypic and Genotypic Detection of Biofilm Formation and Efflux Pump Activity in Multi-drug Resistant Klebsiella Pneumoniae

NCT07541690 · Status: NOT_YET_RECRUITING · Type: OBSERVATIONAL · Enrollment: 100

Last updated 2026-04-21

No results posted yet for this study

Summary

1. Sample: urine, pus and sputum samples.
2. Culture: the samples will be inoculated on the MacConkey agar and subcultured on eosine methylene blue agar.
3. Identification of isolates will be done by:

* Colony morphology.
* Gram staining.
* Biochemical reactions: including Indole, Methyl red, Voges-Proskauer, and Citrate utilization (IMViC) tests.
4. Antimicrobial susceptibility testing of the isolates will be performed using the Kirby-Bauer method (disc diffusion method) using Muller Hinton agar according to the Clinical and Laboratory Standards Institute (CLSI 2025) guidelines for selected groups of antibiotics.
5. Phenotypic detection of biofilm formation by the microtitre plate method.
6. Phenotypic detection of efflux pump activity by ethidium bromide-agar cartwheel method.
7. Detection of biofilm genes (fimH-1, mrkA, and mrkD) by conventional PCR.
8. Detection of efflux pump genes (acrAB, tolC, and mdtk) by conventional PCR.

Conditions

  • Multidrug Resistant Klebsiella Pneumoniae Infection

Interventions

GENETIC

Genotypic Detection of Biofilm Formation and Efflux Pump Activity.

1. Phenotypic detection of biofilm formation by the microtitre plate method. 2. Phenotypic detection of efflux pump activity by ethidium bromide-agar cartwheel method. 3. Detection of biofilm genes (fimH-1, mrkA, and mrkD) by conventional PCR. 4. Detection of efflux pump genes (acrAB, tolC, and mdtk) by conventional PCR.

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Eligibility

Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2026-04-30
Primary Completion
2026-08-31
Completion
2027-02-28

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07541690 on ClinicalTrials.gov