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Impact of Siderophore Genes on Cefiderocol Susceptibility in Carbapenem Resistant Hypervirulent Klebsiella Pneumoniae

NCT07475429 · Status: NOT_YET_RECRUITING · Type: OBSERVATIONAL · Enrollment: 100

Last updated 2026-03-24

No results posted yet for this study

Summary

1. Sample: Clinical specimens (blood, urine, sputum, wound swabs) will be collected aseptically and transported to the microbiology laboratory for analysis.
2. Culture: Specimens will be inoculated on MacConkey agar and incubated at 35-37°C for 18-24 hours. Colonies with typical K. pneumoniae morphology (mucoid, lactose-fermenting) will be selected for further testing
3. Identification of isolates will be done by:

Colony morphology, Gram staining, and conventional biochemical tests (eg, indole, citrate utilization, urease, Voges-Proskauer) .
4. Antimicrobial susceptibility testing of the isolates :

Antimicrobial susceptibility testing was performed and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, M100 document (latest edition)."
5. MIC determination for cifederecol using E-test .
6. Phenotypic Detection of hvKP:

1. String Test: A viscous string ≥5 mm formed by stretching a single colony indicates hypermucoviscosity .
2. Capsule Staining: to visualize large capsules .
3. Siderophore Production: Assessed on chrome azurol S (CAS) agar to detect iron-chelating activity.
7. Detection of siderophore genes by Simple qualitative PCR .

Conditions

  • Carbapenem-resistant Hypervirulent Klebsiella Pneumoniae Infection

Interventions

DIAGNOSTIC_TEST

Cefiderocol susceptibility testing

Determination of cefiderocol susceptibility in carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates using antimicrobial susceptibility testing according to CLSI guidelines.

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Eligibility

Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2026-03-15
Primary Completion
2026-08-01
Completion
2026-10-01

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07475429 on ClinicalTrials.gov