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Type VI Secretion System in Klebsiella Pneumoniae

NCT05516654 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 70

Last updated 2023-04-12

No results posted yet for this study

Summary

Klebsiella pneumoniae (K. pneumoniae) is an important opportunistic pathogen contributing to nosocomial and antimicrobial-resistant infections. The increasing prevalence of infections caused by multidrug-resistant K. pneumoniae has emerged as a major clinical and public health threat, while the serous organ and life-threatening infections caused by highly virulent K. pneumoniae have also emerged( Russo and Marr, 2019; Wyres et al., 2020).

Conditions

  • Detection of Type VI Secretion System in K.Pneumoniae
  • Detection of Relation to Ab Resistance, Biofilm

Interventions

DIAGNOSTIC_TEST

Routine bacteriological cultures

culture of different samples on different culture media as nutrient agar, blood agar, MacConkey agar, EMB agar, TSI

DIAGNOSTIC_TEST

Identification of the organism and antibiotic sensitivity will be done by VITEK II

Automated identification of the organism and antibiotic sensitivity will be done by VITEK II

DIAGNOSTIC_TEST

Detection of biofilm formation

Detection of Biofilm formation to the collected organism by tissue culture plate method.

DIAGNOSTIC_TEST

Molecular detection by PCR

Molecular detection of Type VI Secretion System genes, capsular serotyping( k1, k2), and FimH genes by PCR.

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Principal Investigators

  • Noha S Shfik, lecturer · Faculty of medicine, Sohag university

Eligibility

Min Age
12 Weeks
Max Age
90 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2022-09-10
Primary Completion
2023-01-30
Completion
2023-09-30

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05516654 on ClinicalTrials.gov