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Evaluation of the Relationship Between Oxidative Stress and Human Reproduction

NCT05575739 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 80

Last updated 2022-10-12

No results posted yet for this study

Summary

The aim of the study is to investigate the role of oxidative stress in female infertility and the relationship between the stress as it is illustrated by questionnaires, serum markers, follicular fluid markers, and ovarian response in assisted reproduction.

Conditions

  • IVF
  • Pregnancy
  • Oxidative Stress
  • Fertility Disorders

Interventions

DIAGNOSTIC_TEST

Measurement of oxidative stress levels (Malondialdehide, glutathione disulphate, total antioxidant capacity, superoxide dismutase and carbonylated proteins )

Follicular fluid and serum were obtained within 1 hour of oocyte recovery. Considerable care was taken to pool follicular fluid uncontaminated with blood. The samples were processed by centrifuge and 1-mL aliquots stored ar -80ºC until assay. Malondialdehide, glutathione disulphate, total antioxidant capacity, superoxide dismutase and carbonylated proteins were measured from follicular fluid and plasma according to manufacturer's protocol. This assay has been described as highly sensitive. Data were expressed as millimoles per liter from the reference curve.

Sponsors & Collaborators

  • Calla IVF Center

    lead OTHER

Principal Investigators

  • Anca Huniadi · CALLA IVF

Eligibility

Min Age
20 Years
Max Age
54 Years
Sex
FEMALE
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-02-14
Primary Completion
2022-12-31
Completion
2023-02-14

Countries

  • Romania

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05575739 on ClinicalTrials.gov