Cryotherapy on Inflammatory Profile and Postoperative Pain Levels in Endodontic Infections

Summary

The aim of this study is to evaluate the effects of cryotherapy applications on the inflammatory cytokine and collagenase matrix metalloproteinase levels during root canal treatment and postoperative pain intensity and incidence. Mandibular premolar teeth of 60 male patients within the 20-30 years old range, diagnosed with asymptomatic apical periodontitis will be included to the study for this purpose. The experimental protocols consist clinical and laboratory phases. In clinical phase, procedures of cryotherapy and control groups will be applied in 2-visit-root canal treatment. The samples, which were collected during root canal treatment, will be subjected to enzyme-linked immunosorbent assay (ELISA) analysis in laboratory. Levels of interleukin and inflammatory destructive enzymes will be determined in collected samples. During the analysis of visual analogue scale scores, the correlation between the changes of the cytokine and proteolytic enzyme levels and presence and intensity of pain will be evaluated.

Conditions

• Apical Periodontitis
• Postoperative Pain
• Cytokine Levels

Interventions

DEVICE

Distilled water irrigation kept at 2.5°C degree

The use of cold distilled water for final irrigation of root canal system during root canal treatment

DEVICE

Distilled water irrigation kept at room temperature

The use of distilled water kept at room temperature for final irrigation of root canal system during root canal treatment

Sponsors & Collaborators

• The Scientific and Technological Research Council of Turkey

  collaborator OTHER

• Ondokuz Mayıs University

  lead OTHER

Principal Investigators

• Elif Kalyoncuoğlu, PhD · Ondokuz Mayıs University

• Abdurrahman Aksoy, PhD · Ondokuz Mayıs University

• Ayşegül Arkan, DDS · Ondokuz Mayıs University

• Onur Kömeç, DDS · Ondokuz Mayıs University

• Elçin Yüzgüleç, DDS · Ondokuz Mayıs University

• Hande Akgün, DDS · Ondokuz Mayıs University

• Sabiha G Afak, DDS · Ondokuz Mayıs University

Study Design

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

DOUBLE

Model

PARALLEL

Eligibility

Min Age

20 Years

Max Age

30 Years

Sex

MALE

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2020-11-15

Primary Completion

2022-03-31

Completion

2022-04-15

Countries

• Turkey (Türkiye)

Study Locations