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Role of LncRNA H19 in The Regulation of IGF-1R Expression

NCT04767750 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 101

Last updated 2022-04-07

No results posted yet for this study

Summary

Hepatocellular carcinoma (HCC) is a common cancer that poses a heavy economic burden on the healthcare system. In Egypt, it is the most common cause of mortality and morbidity-related cancer. Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia. Cancer and type II diabetes (T2DM), the world's two most prevalent diseases, share many overlapping risk factors and predisposing pathological conditions. The exact mechanisms linking those two diseases are yet to be fully understood.

In this study, the investigators aim to assess the relationship between Long Non-Coding RNA (lncRNA) H19 and Insulin-Like Growth Factor 1 Receptor (IGF-1R) mRNA gene expressions in the blood samples of HCC \& T2DM patients to investigate the probability of the presence of a pathophysiological link between HCC and DM that may become a therapeutic target for both diseases. To the investigator's knowledge, there is currently no human research study investigating both H19 and IGF-1R in both DM and cancer.

Conditions

Interventions

OTHER

Blood sample collection

5ml of peripheral blood samples will be collected into tubes containing EDTA from all subjects and the following procedures will be performed: * Peripheral blood mononuclear cells (PBMCs) will be isolated from blood samples. * Total RNA extraction from PBMCs by Trizol reagent and spin column. * Reverse transcription to cDNA. * The expression levels of lncRNA H19, IGF-1R mRNA and GAPDH gene (control gene) will be determined by Real-Time Quantitative PCR

Sponsors & Collaborators

  • Mansoura University

    lead OTHER

Principal Investigators

  • Noura MS Shehabeldin, M.B.B.Ch · Faculty of Medicine, Mansoura University.

  • Mohamed AA Zahran, M.D. · Faculty of Medicine, Mansoura University.

  • Heba K Mohamed, M.D. · Faculty of Medicine, Mansoura University.

  • Nora M Hussein, M.D. · Faculty of Medicine, Mansoura University.

  • Ahmed Shehta, M.D. · Gastroenterology Center, Mansoura University

  • Helmy Ezzat, M.D. · Gastroenterology Center, Mansoura University

Eligibility

Min Age
40 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2020-01-01
Primary Completion
2022-01-30
Completion
2022-04-04

Countries

  • Egypt

Study Locations

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04767750 on ClinicalTrials.gov