Interaction Between Immune Cells and Bacteria Associated With Periodontitis

Summary

This study evaluates the interaction between host immune cells and bacteria associated with periodontitis. It comprises biological material from donors with and without periodontal disease. Specifically, we collect a spit and blood sample to conduct in vitro stimulations and measurements of selected parameters related to periodontitis to clarify obscure areas in the immunologic pathogenesis of this disease.

Conditions

• Periodontal Diseases
• Periodontitis
• Aggressive Periodontitis
• Immunologic Disease
• Microbial Disease
• Periodontal Pocket
• Inflammation
• Inflammation Gum
• Dysbiosis
• Rheumatoid Arthritis
• Generalized Aggressive Periodontitis
• Generalized Chronic Periodontitis
• Chronic Periodontitis

Interventions

OTHER

In vitro stimulation of blood with periodontitis-associated- and control bacteria

Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells.

DIAGNOSTIC_TEST

Anti-CCP- and anti-P.g.-antibodies titers

Anitbody titers will be measured in saliva and serum samples.

GENETIC

Analysis of selected single nucleotide polymorphisms (SNPs)

DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs.

DIAGNOSTIC_TEST

periodontitis-associated bacteria presence

Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.

Sponsors & Collaborators

• Copenhagen University Hospital, Denmark

  collaborator OTHER

• University of Copenhagen

  lead OTHER

Principal Investigators

• Palle Holmstrup, DDS, PhD, Dr Odont · University of Copenhagen

• Claus Henrik Nielsen, PhD, MSc, MD · Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Eligibility

Min Age

19 Years

Max Age

60 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2017-02-01

Primary Completion

2018-10-12

Completion

2020-03-23

Countries

• Denmark

Study Locations