The Effects of Acute Total Sleep Deprivation Versus Normal Sleep on Metabolism

Summary

The study proposes to investigate whether acute total deprivation affects metabolism as measured through blood and peripheral tissues. Its aim is also to investigate how acute total sleep deprivation affects neurodegenerative markers, as well as hormones, memory performance and aspects of appetite regulation.

Conditions

• Sleep Deprivation
• Sleep

Interventions

BEHAVIORAL

Inhibitory task

Participants perform a binary decision on each presented stimuli. Of the two possible outcomes, participants are instructed to make a motor response (go) for one type, and are to withhold a response (no-go) for the other type. Reaction time and accuracy are measured for each event

PROCEDURE

Blood samples

Hormone levels, neuromolecular levels and gene expression profiles will be analyzed from repeated blood samples obtained before and after the nighttime intervention

PROCEDURE

Tissue samples

Expression profiles will be analyzed from samples obtained from tissues involved in metabolism

PROCEDURE

Oral glucose tolerance test

75 g of glucose will be dissolved in 300 ml of water and given to participants, followed by blood sampling at 0, 15, 30, 60, 90, 120 and 150 minutes following the ingestion of the glucose solution.

BEHAVIORAL

Portion Size Task

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Sponsors & Collaborators

• Uppsala University

  lead OTHER

Principal Investigators

• Christian Benedict, PhD · Department of Neuroscience, Uppsala University

Study Design

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Model

CROSSOVER

Eligibility

Min Age

18 Years

Max Age

28 Years

Sex

MALE

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2013-03-31

Primary Completion

2013-10-31

Completion

2013-10-31

Countries

• Sweden

Study Locations