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Genes Associated With Bone Metabolism in the Saliva During Orthodontic Treatment

NCT07303647 · Status: ACTIVE_NOT_RECRUITING · Type: OBSERVATIONAL · Enrollment: 24

Last updated 2025-12-26

No results posted yet for this study

Summary

Understanding the biological events during fixed orthodontic treatment is essential for optimizing treatment strategies, predicting patient response, and minimizing adverse effects. Most studies on bone remodeling have used invasive sampling methods such as tissue biopsies or serum collection; these methods cannot be used for routine clinical monitoring. Saliva is a simple medium that can reflect changes in local periodontal and bone conditions, it is also non-invasive and cheap. There is little evidence about the temporal expression of genes related to bone metabolism (RANKL, OPG, ALP, TRAP, RUNX2) in saliva during orthodontic therapy. This study will help advance the understanding of biological responses during orthodontic tooth movement and explore whether saliva can be an appropriate diagnostic medium for monitoring bone remodeling in orthodontic patients

Conditions

  • Gene Expression Profiling
  • Orthodentic Appliances

Interventions

DIAGNOSTIC_TEST

PCR

Unstimulated whole saliva collection SOP (time of day, fasting, avoid toothbrushing immediately prior). RNA stabilization and extraction (saliva RNA kits). cDNA synthesis and quantitative RT-PCR (or RNA-seq if budget allows). Housekeeping genes for normalization (e.g., GAPDH, ACTB - validate stability in saliva). Analysis method: ΔΔCt → fold change.

Sponsors & Collaborators

  • Kurdistan Higher Council of Medical Specialties

    lead OTHER

Eligibility

Min Age
15 Years
Max Age
25 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2025-10-12
Primary Completion
2026-02-28
Completion
2026-02-28

Countries

  • Iraq

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07303647 on ClinicalTrials.gov