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Effects of Acute Energy Deficit and Aerobic Exercise on Muscle Quality

NCT05203133 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 10

Last updated 2022-11-09

No results posted yet for this study

Summary

10 healthy, male, participants will complete a a 5-day baseline assessment (days -5 to -1) and two consecutive 5-day periods of controlled exercise to increase oxidative capacity (3 days of aerobic exercise per period, 15 kcal/kg FFM/day energy expenditure cycling) and energy intake (15 days in total, with a testing session on morning 16). This will achieve states of energy balance (EB; energy availability - EA - 45 kcal/kg of fat free mass (FFM)/day), required for weight maintenance (days 1 - 5), followed by energy deficit (ED; EA 10 kcal/kg FFM/day), required for weight loss on days 6 - 10.

Over the data-collection period, participants will consume deuterium (D2O) tracer to facilitate dynamic proteomic profiling to assess the impact of the intervention on muscle quality (primary outcome measure). Muscle biopsies will therefore be collected on days -5, 1, 6 \& 11, alongside daily saliva samples, and venous blood collection on days -5, 1, 3, 5, 6, 8, 10 \& 11. These samples will be used to assess further, secondary, outcome measures including alterations in intra-muscular lipid profiles (lipid droplet content, morphology and lipid-droplet associated proteins in different subcellular compartments \[intermyofibrillar vs subsarcolemmal\]), alterations in blood metabolites and hormones and skeletal muscle glycogen concentrations. Changes in body mass, body composition and RMR will also be assessed.

Conditions

  • Energy Supply; Deficiency
  • Energy Balance
  • Energy Availability

Interventions

DIETARY_SUPPLEMENT

Energy Balance

Energy balance phase to elicit weight-maintenance

DIETARY_SUPPLEMENT

Energy Deficit

Energy deficit phase to elicit weight-loss

DIETARY_SUPPLEMENT

Baseline Assessment

Free-living assessment of energy status

Sponsors & Collaborators

  • Alliance for Potato Research and Education

    collaborator OTHER

  • Liverpool John Moores University

    lead OTHER

Principal Investigators

  • Jose Areta, PhD · Liverpool John Moores University

Study Design

Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Model
SEQUENTIAL

Eligibility

Min Age
18 Years
Max Age
40 Years
Sex
MALE
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-08-23
Primary Completion
2021-12-15
Completion
2021-12-15

Countries

  • United Kingdom

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05203133 on ClinicalTrials.gov