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Diagnostic Accuracy of Salivary DNA Integrity Index in Oral Malignant and Premalignant Lesions

NCT03682562 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 93

Last updated 2020-07-15

No results posted yet for this study

Summary

This study aims to identify the accuracy of DNA integrity index in differentiating between oral premalignant lesions and oral cancer.

Conditions

  • Oral Cancer
  • Oral Lichen Planus
  • Oral Leukoplakia

Interventions

DIAGNOSTIC_TEST

DNA Integrity Index

This test will be performed for ALL three groups: Sample collection: Patients will be given a sterile cup containing 5mL saline solution with which they will vigorously rinse their mouth while rubbing their tongues against the oral mucosa for 30 seconds, then spit it back into the cup. The solution will then be used for DNA extraction. DNA integrity analysis: The DNA integrity will be done through measuring a housekeeping gene using competitive polymerase chain reaction where the isolated DNA will be combined with DNA capture probes for sequence-specific DNA fragments. The DNA integrity index will be calculated as the ratio of the concentration of longer DNA fragments to the ratio of shorter ones.

Sponsors & Collaborators

  • Cairo University

    lead OTHER

Eligibility

Min Age
20 Years
Max Age
70 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2019-09-15
Primary Completion
2020-03-10
Completion
2020-03-10

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT03682562 on ClinicalTrials.gov