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Design of a Protocol for Obtaining Genomic DNA From Saliva

NCT02523326 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 60

Last updated 2015-08-14

No results posted yet for this study

Summary

Objective The aim of this study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease.

Materials and methods Saliva samples were taken from 60 patients, then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.

Conditions

  • Periodontal Disease "Unrecognized Condition"

Interventions

DEVICE

saliva samples obtained with mouthwash

saliva samples obtained with mouthwash

Sponsors & Collaborators

  • Centro Interdisciplinario de Ciencias de la Salud Unidad Santo Tomás

    lead OTHER

Principal Investigators

  • Beatriz Buentello Volante, Doctorate · Instituto de Oftalmología "Conde de Valenciana".

  • María Esther Ocharán Hernández, Doctorate · Escuela Superior de Medicina, Instituto Politécnico Nacional

  • Claudia Camelia Claudia Camelia Calzada Mendoza, Doctorate · Escuela Superior de Medicina, Instituto Politécnico Nacional

  • Arturo Flores Pliego, Doctorate · Instituto Nacional de Perinatología

  • Héctor A. Baptista Gonzalez, Doctorate · Instituto Nacional de Perinatología

  • Higinio Estrada Juárez, Doctorate · Instituto Nacional de Perinatología

Eligibility

Min Age
35 Years
Max Age
45 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2015-02-28
Primary Completion
2015-08-31
Completion
2015-09-30

Countries

  • Mexico

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT02523326 on ClinicalTrials.gov