Human Muscle Gene Expression Profiles in Young and Old Men

Summary

To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.

The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.

The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.

Conditions

• Elderly
• Sarcopenia

Interventions

OTHER

Skeletal muscle aging

W1 - Blood test * Maximal voluntary contraction of quadriceps * Hydrostatic weighing W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy

Sponsors & Collaborators

• Fondation des Caisses d'Epargne Rhône-Alpes

  collaborator UNKNOWN

• Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

  collaborator OTHER

• Centre Hospitalier Universitaire de Saint Etienne

  lead OTHER

Principal Investigators

• Daniel BECHET, PhD · INRA Clermont-Ferrand

• Jean-Claude BARTHELEMY, MD PhD · CHU de Saint-Etienne

Eligibility

Min Age

19 Years

Max Age

76 Years

Sex

MALE

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2008-10-31

Primary Completion

2009-03-31

Completion

2009-03-31

Countries

• France

Study Locations