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Gene Expression Analysis of Biofilm Formation and Antimicrobial Resistance in Clinical Methicillin-Resistant Staphylococcus Aureus

NCT07796555 · Status: NOT_YET_RECRUITING · Type: OBSERVATIONAL · Enrollment: 100

Last updated 2026-09-01

No results posted yet for this study

Summary

Staphylococcus aureus are Gram-positive cocci that asymptomatically colonize the skin and mucosal surfaces of approximately 20-30% of healthy individuals . Although they commonly exist as a harmless commensal organisms, S. aureus are one of the most clinically significant opportunistic pathogens, causing a wide range of community and healthcare-associated infections. These infections vary from superficial skin and soft tissue infections to severe invasive diseases, including bacteremia, infective endocarditis, pneumonia, osteomyelitis, and device-associated infections .

Conditions

  • Staphylococcal Aureus Infection
  • Surgical Site Infection (SSI)

Interventions

DIAGNOSTIC_TEST

swab from lesion

* Direct smear with Gram stain to identify the morphology of Staphylococcus aureus. * culture of the samples on different culture media such as nutrient agar, blood agar, and Mannitol salt agar.

DIAGNOSTIC_TEST

Biochemical reactions

biochemical reactions: catalase test will be done to confirm diagnosis of Staphylococcus aureus.

DIAGNOSTIC_TEST

Antibiotic sensitivity

Antibiotic sensitivity test will be done using different antibiotics according to CLSI 2026.

DIAGNOSTIC_TEST

Molecular detection of antibiotic resistance

Assessment of the expression of selected biofilm-related genes (icaA and fnbA) and antibiotic resistance-related genes (mecA and femA) using quantitative real-time PCR (qRT-PCR) before and after treatment with probiotic cell-free supernatant (CFS).

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Eligibility

Min Age
6 Months
Max Age
90 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2026-09-20
Primary Completion
2027-08-30
Completion
2027-11-02

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07796555 on ClinicalTrials.gov