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Effects of Ozone Therapy on Muscle Damage

NCT07728422 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 32

Last updated 2026-07-27

No results posted yet for this study

Summary

The goal of this clinical trial is to determine the effectiveness of different ozone therapy methods in enhancing recovery after muscle-damaging exercise in healthy adults, and to evaluate their impact on biochemical markers of muscle damage and fatigue.

The main questions it aims to answer are:

* Do ozone-based interventions accelerate recovery by reducing biochemical markers of muscle damage and fatigue following eccentric exercise?
* Which ozone therapy modality provides the greatest recovery benefit? Researchers will compare three intervention groups (autohemotherapy, ozone sauna therapy, and ozonated lavender oil massage) with a control group receiving no treatment.

Participants will:

* Complete a standardized eccentric exercise protocol designed to induce muscle damage
* Receive one of the assigned interventions or no treatment (control)
* Provide blood samples at multiple time points (baseline, post-exercise, post-intervention, and 24, 48, and 72 hours follow-up) to assess recovery- related biomarkers

Conditions

  • Ozone Therapy
  • Muscle Damage
  • Oxidative Stress
  • Recovery

Interventions

OTHER

Autohemotherapy

Autohemotherapy was performed by withdrawing 100 mL of venous blood, which was subsequently exposed to an oxygen-ozone gas mixture (5% ozone, 95% oxygen) for 5-10 minutes in an ozone-resistant, sterile container. The ozonated blood was then rein-fused intravenously over a 15-minute period in accordance with established clinical protocols

OTHER

Ozone Sauna Therapy

Ozone sauna therapy was administered in a sealed cabin maintained at a tempera-ture of 45°C. Participants remained seated with the head positioned outside the cabin and the neck sealed using a polyethylene collar to prevent ozone inhalation. Each session lasted 20 minutes and was conducted at an ozone concentration of 20 µg·mL-¹

OTHER

Ozonated Lavender Oil Massage

Massage with ozonated lavender oil was administered by a certified massage thera-pist and targeted the lower extremity muscle groups, including the quadriceps, ham-strings, and gluteal muscles. The intervention lasted 20 minutes in total (10 minutes per limb) and followed standardized techniques adapted from Braun and Simonson (2008).

Sponsors & Collaborators

  • Inonu University

    lead OTHER

Study Design

Allocation
RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Model
PARALLEL

Eligibility

Min Age
18 Years
Max Age
30 Years
Sex
MALE
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2024-10-04
Primary Completion
2024-11-28
Completion
2025-02-20

Countries

  • Turkey (Türkiye)

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07728422 on ClinicalTrials.gov