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Bacterial Profile Changes During Different Sodium Hypochlorite Irrigation Regimens and Calcium Hydroxide Medicament in Root Canal Treatment

NCT07623278 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 24

Last updated 2026-06-05

No results posted yet for this study

Summary

The goal of this clinical trial is to learn how the bacterial profile in infected root canals changes during different phases of routine root canal treatment in adults with chronic apical abscess. The main questions it aims to answer are:

* How does the root canal bacterial profile change before and after irrigation with 5.25 percent sodium hypochlorite or 3 percent sodium hypochlorite?
* How does the bacterial profile change after calcium hydroxide medicament is placed in the root canal?

Participants will:

* Receive standard root canal treatment with one of two irrigation regimens (5.25 percent or 3 percent sodium hypochlorite, plus EDTA),
* Have root canal samples taken three times (before any treatment, after irrigation, and after calcium hydroxide medicament), and
* Have the bacteria in these samples analyzed using 16S rRNA PCR and DNA sequencing.

Conditions

  • Chronic Apical Abscess
  • Root Canal Infection

Interventions

PROCEDURE

Root canal treatment with 5.25% sodium hypochlorite irrigation

Standard root canal procedure in which the canal is irrigated with 5.25 percent sodium hypochlorite, followed by sterile distilled water and 17 percent EDTA, then calcium hydroxide intracanal medicament and final obturation, with bacterial sampling at three time points.

PROCEDURE

Root canal treatment with 3% sodium hypochlorite irrigation

Standard root canal procedure in which the canal is irrigated with 3 percent sodium hypochlorite, followed by sterile distilled water and 17 percent EDTA, then calcium hydroxide intracanal medicament and final obturation, with bacterial sampling at three time points.

Sponsors & Collaborators

  • Hasanuddin University

    lead OTHER

Principal Investigators

  • Beatrix Zitzy Uniquen Enoch, DDS · Dental and Oral Hospital Hasanuddin University

  • Prof. Maria Tanumihardja, DDS, MDS · Dental and Oral Hospital Hasanuddin University

Study Design

Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Model
PARALLEL

Eligibility

Min Age
18 Years
Max Age
75 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2025-10-13
Primary Completion
2026-03-30
Completion
2026-05-18

Countries

  • Indonesia

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07623278 on ClinicalTrials.gov