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Circadian Clock Gene Expression in Periodontal Disease

NCT07452783 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 60

Last updated 2026-03-06

No results posted yet for this study

Summary

This observational study aims to investigate whether periodontal inflammation is associated with alterations in the expression of circadian clock-related genes and proteins in gingival tissues. Circadian rhythms regulate many biological processes, including immune responses and inflammation. Although experimental studies suggest a link between circadian disruption and periodontal disease, human data under controlled chronotype conditions are limited.

A total of 60 systemically healthy, non-smoking individuals aged 22-45 years with comparable sleep patterns (intermediate chronotype and 6-9 hours of sleep) were included. Participants were classified as periodontally healthy, gingivitis, or stage III grade B periodontitis according to established diagnostic criteria. Gingival tissue samples were collected during clinically indicated procedures within a standardized morning time window (09:00-11:00).

Gene expression levels of circadian clock components (CLOCK, BMAL1, PER1-3, CRY1-2, Rev-Erb-β, ROR-α) and inflammatory mediators (IL-1β, IL-6, TNF-α, NF-κB, IFN-γ, RANKL, OPG) were analyzed using RT-qPCR, Western blot, and ELISA techniques. Associations between molecular findings and clinical periodontal parameters were evaluated.

The study seeks to clarify whether periodontal disease itself may disrupt local circadian regulatory mechanisms in gingival tissues.

Conditions

  • Gingivitis
  • Periodontitis

Interventions

PROCEDURE

Gingival Tissue Biopsy

Collection of gingival tissue samples from interproximal sites during clinically indicated periodontal procedures for molecular and protein expression analyses.

Sponsors & Collaborators

  • Inonu University

    lead OTHER

Principal Investigators

  • Cuneyt A Aral, Professor, DDS, PhD · Inonu University

Eligibility

Min Age
25 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2024-01-20
Primary Completion
2025-03-02
Completion
2025-12-01

Countries

  • Turkey (Türkiye)

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07452783 on ClinicalTrials.gov