PRF and CGF Scaffolds With EDTA in Regeneration

Summary

This study evaluated the regenerative outcomes of immature permanent teeth with necrotic pulps treated using three scaffold types-blood clot, platelet-rich fibrin (PRF), and concentrated growth factor (CGF)-combined with either 5% or 17% EDTA as the final irrigant. Thirty teeth were randomly assigned to six treatment groups and followed clinically and radiographically for 12 months. Cone-beam computed tomography (CBCT) was used to assess root development parameters, including root length increase, apical diameter change, hard tissue formation, and periapical lesion volume. Clinical assessments included pain, swelling, sinus tract formation, percussion sensitivity, and pulp sensitivity testing. The study aimed to determine whether different scaffold materials and EDTA concentrations influence regenerative healing responses in immature necrotic teeth.

Conditions

• Pulp Necrosis
• Immature Permanent Teeth
• Open Apex
• Periapical Periodontitis
• Procedure

Interventions

PROCEDURE

Blood Clot + 5% EDTA

Final irrigation with 5% EDTA followed by induction of apical bleeding to form an intracanal blood clot scaffold as part of the regenerative endodontic procedure.

PROCEDURE

Blood Clot + 17% EDTA

Final irrigation with 17% EDTA followed by controlled induction of bleeding beyond the apex to produce a blood clot scaffold for regenerative endodontic treatment.

PROCEDURE

PRF + 5% EDTA

Application of platelet-rich fibrin (PRF) scaffold after final irrigation with 5% EDTA during regenerative endodontic therapy.

PROCEDURE

PRF + 17% EDTA

Use of platelet-rich fibrin (PRF) scaffold following final irrigation with 17% EDTA in regenerative endodontic treatment.

PROCEDURE

CGF + 5% EDTA

Placement of concentrated growth factor (CGF) scaffold after final irrigation with 5% EDTA as part of the regenerative endodontic protocol.

PROCEDURE

CGF + 17% EDTA

Placement of concentrated growth factor (CGF) scaffold following final irrigation with 17% EDTA during regenerative endodontic treatment.

Sponsors & Collaborators

• Kahramanmaras Sutcu Imam University

  lead OTHER

Principal Investigators

• Aliye Kamalak · Kahramanmaraş Sütçü İmam University, Faculty of Dentistry, Department of Endodontics

Study Design

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

NONE

Model

PARALLEL

Eligibility

Min Age

9 Years

Max Age

25 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2023-10-01

Primary Completion

2024-10-01

Completion

2024-10-01

Countries

• Turkey (Türkiye)

Study Locations