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High-fat Diet (HFD) and Small Intestinal Microbiome

NCT07019142 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 10

Last updated 2025-06-13

No results posted yet for this study

Summary

The investigators aim to characterize the upper small intestinal microbiome in healthy conditions in dependency on feeding condition (starved vs. high-fat-challenged) and diet (before and after 3 day high-fat-diet)

Primary outcome will be the maximum culturable bacteria (aerobic and anaerobic) in small intestinal luminal content during acute liquid-fat challenge before vs. after a short-term (3 day) high-fat-diet

In this pilot study, healthy volunteers will undergo i) an upper endoscopy for harvesting of small intestinal juice, mucosal biopsies and ii) blood withdrawal at baseline (starved) as well as during acute one-time high-fat luminal stimulation (within 3 hours after baseline) utilizing microbiological culture and 16S-RNA sequencing. The same procedure will be performed after 3 day high-fat-diet.

During endoscopy an acute luminal high-fat challenge which will be repeated after a short-term (3 day) high-fat diet

Conditions

  • Microbial Colonization
  • Small Intestinal Bacterial Overgrowth

Interventions

DIETARY_SUPPLEMENT

HFD

Luminal challenge with standardized volume, amount, calories of fat

Sponsors & Collaborators

  • Insel Gruppe AG, University Hospital Bern

    lead OTHER

Study Design

Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Model
SINGLE_GROUP

Eligibility

Min Age
18 Years
Max Age
80 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-03-01
Primary Completion
2024-05-31
Completion
2024-11-01

Countries

  • Switzerland

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07019142 on ClinicalTrials.gov