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Cutaneous Microbiota Evolution in ICU Patients With CVC (ICMc)

NCT06095076 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 120

Last updated 2023-10-23

No results posted yet for this study

Summary

Intensive Care Unit (ICU) patients are exposed to catheter-related infections with an important morbidity. Catheter colonization is constant but infection is not. Cutaneous dysbiosis could be the missing link. Our study aims to evaluate the evolution of cutaneous microbiota in ICU patients with a central venous catheter in place, through metagenomics. Our main objective is to evaluate the evolution of alpha-diversity, quantified by intra-patient variation of Shannon diversity index (a diversity index used in bacterial metagenomics).

Conditions

Interventions

DIAGNOSTIC_TEST

Skin swabbing

Skin swabbing will be performed on CVC insertion site before CVC placement (baseline), and then every 3 days (or when dressing is changed) while CVC is in place, then at ICU discharge. Bacterial metagenomics using bacterial DNA extraction, 16S PCR amplification and Nanopore sequencing will allow for description of cutaneous microbiota and diversity evaluation through Shannon index. Evolution of alpha-diversity will be evaluated through time-series data analysis: comparison of Shannon index at various time points with baseline Shanonn index (before CVC placement). Standard microbiologic culture of skin swabbing will be performed.

OTHER

Data collection

General patient characteristics and informations relative to CVC infection and treatment will be collected.

Sponsors & Collaborators

  • Tropical Biome and ImmunoPhysiopathology (TBIP) - French Guiana University (Université de Guyane)

    collaborator UNKNOWN

  • Centre Hospitalier de Cayenne

    lead OTHER

Principal Investigators

  • Ariane ROUJANSKY · Centre Hospitalier de Cayenne

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2023-06-02
Primary Completion
2023-12-02
Completion
2024-06-02

Countries

  • French Guiana

Study Locations

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT06095076 on ClinicalTrials.gov