Fetal Cell Receptors Repertoire

Summary

The purpose of this study is to describe the transcriptomic profile of foetal cells in post-partum and more specifically to determine which chemokine receptors are overexpressed in foetal cells in post-partum women with wounds To do so, the investigators will isolate foetal cells from the peripheral blood of healthy controls post partum women as well as from post partum women with skin ulcers and then perform RNA sequencing.

Conditions

• Venous Ulcer
• Sickle Cell Ulcer
• Diabetic Ulcer
• Post-partum Women
• Mixed Ulcer

Interventions

OTHER

Saliva sampling

HLA genotyping. The technique should allow to identify, for children's, a paternal HLA antigen not shared with the mothers.

OTHER

Blood sampling

Maternal Blood samples will be incubated with the appropriate antibody, targeting the microchimeric fetal cells of each patient, as well as with a cell viability marker (DAPI). The samples were then be processed through the BD FACS Aria III to sort the fetal cells, The following steps - RNA extraction, quality control, retrotranscription, preparation of the library, sequencing and transcriptomic analysis - will be carried out according to the Smart-seq3 protocol. The data will be sent for in-depth analysis and confirmation of the results. Additional functional experiments may also be carried out.

OTHER

Interviews

V2 and/or V3

OTHER

Clinical examination

V2 and/or V3

Sponsors & Collaborators

• URC-CIC Paris Descartes Necker Cochin

  collaborator OTHER

• Assistance Publique - Hôpitaux de Paris

  lead OTHER

Principal Investigators

• Sélim ARACTINGI, MD, PHD · Dermatology unit, Cochin Hospital - APHP

Study Design

Allocation

NON_RANDOMIZED

Purpose

OTHER

Masking

NONE

Model

PARALLEL

Eligibility

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2024-02-06

Primary Completion

2026-07-01

Completion

2027-02-01

Countries

• France

Study Locations