Phenotypic and Genotypic Variations of Enterococcus Spp

Summary

Enterococci are Gram-positive facultative anaerobic cocci arranged in short and medium chains. Enterococci reside in the gastrointestinal tract and usually function commensally with humans. They can, however, cause several infections, such as urinary tract infections (UTIs), intra-abdominal infection, bacteremia, or endocarditis.

Among many species identified, E. faecalis and E. faecium are the most common species capable of causing infection and posing a threat of antimicrobial resistance, with E. faecalis accounting for the majority of infections.

Conditions

• Isolation of Enterococci From Samples
• Demonstration of Enterococci Antibiotic Susceptibility
• Detection of Strains Enterococci Produce Biofilm
• Studying Effect of Nanoparticles on Enterococci Antimicrobial Resistance
• Studying Effect of Nanoparticles on Enterococci Biofilm
• Genotyping of Enterococci

Interventions

DIAGNOSTIC_TEST

Culture

* Samples will be transported to Medical Microbiology and Immunology Laboratory and will be inoculated on MacConkey medium. * Pink colonies will be inoculated on bile esculin agar medium. * Enterococci produce blackening of the agar

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Staining:

The growing colonies on bile esculin agar medium will be examined microscopically after staining by Gram stain.

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Biochemical reactions:

* Catalase test. * Salt tolerance test

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VITEK

Automated identification of Enterococci will be done with VITEK2 system.

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Antibiotic sensitivity test

Strains confirmed as Enterococci will be examined for their antibiotic susceptibility by modified Kirby Bauer's disc diffusion method on Mueller-Hinton Agar.

DIAGNOSTIC_TEST

nanoparticles effect

The effect of nanoparticles on Enterococci antimicrobial resistance pattern

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The biofilm formation activity

The biofilm formation activity of Enterococci isolates will be tested using the microtiter plate technique

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molecular diagnosis

Molecular identification of some virulence factors genes and antibiotic resistance genes of Enterococci using PCR

Sponsors & Collaborators

• Sohag University

  lead OTHER

Principal Investigators

• Mona F Mohamed, professor · Sohag University

• Wesam A Abu El wafa · Sohag University

Eligibility

Min Age

1 Month

Max Age

90 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2023-04-01

Primary Completion

2023-12-31

Completion

2024-03-30

Countries

• Egypt

Study Locations