Effects of Allium Sativum and Moringa Oleifera Extract on Dental Enamel

Summary

Primary objective is to observe the effects of lead on dental enamel of rabbit by measuring;

* Structural and morphological change by SEM, AFM
* Elemental composition by SEM-EDX
* Molecular composition and hydroxyapatite crystal changes by Raman Spectroscopy
* Blood parameters by serum analysis Secondary objectives includes
* To compare the protective effects of Allium sativum and Moringa oleifera on dental enamel defects due to lead
* To determine the beneficial effects of AS and MO extract in remineralization of dental enamel.

Conditions

• Lead Poisoning

Interventions

OTHER

Lead Acetate

The oral LD50 of lead acetate is 390mg/kg body weight. The experimental group B, C, and D will receive a single dose of 19.5 mg/kg b.w (1/120th of LD50),31 daily dissolved in distilled water and given by oral gavage method for 12 weeks. Lead acetate Pb(CH3COO)2x3H2O, will be purchased from Sigma-Aldrich Chemie (Germany).

DIETARY_SUPPLEMENT

Moringa oleifera Extract

The method described by Hanaa Elgamily(32) with minor modifications. Fresh Leaves will be washed and dried in dry heat oven. All the preparation will be done in pharmacological department, University of Health Sciences in sterile environment. Dried leaves will be crushed to make fine powder. 1000 g powder of MO will be socked in 5000 ml of 70% ethanol (70% ethanol and 30% distilled water) for 72 hours at room temperature. Then mixture will be magnetically stirred for 24 hours and stored at 4oC for 48 hours to allow extraction of active ingredients. Then the mixture will be filtered twicely by using Whatman No 1 filter paper. The extract will be concentrated by rotary vacuum evaporator at 40 oC to evaporate the solvent. The extract will be freeze, dried and stored at -70 oC for use. Dose of MO extract will be 400 mg per kg.(33) body weight given daily in 1ml of distilled water by oral gavage method for 12 weeks.

DIETARY_SUPPLEMENT

Allium sativum Extract

Fresh Allium sativum bulbs will be selected for study. The cloves will be peeled, washed with distilled water and dried under shed. Then it will be chopped into small pieces. 1000g of chopped garlic will be crushed by mortar and passel. The garlic paste will be mixed with 5000 ml of 70% ethanol (70% ethanol and 30% distilled water) for 72 hours at room temperature. Rest the procedure is same as mentioned in moringa extract. Dose of 500 mg per kg(34) body weight garlic extract will be given daily in 1ml of distilled water.

OTHER

Placebo

1ml NaCl 0.9% daily for 12 weeks

Sponsors & Collaborators

• Baqai Medical University

  collaborator OTHER

• Arslan Saleem

  lead OTHER

Principal Investigators

• Rashid Javaid, Ph.D scholar · Baqai Medical University

Study Design

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

DOUBLE

Model

FACTORIAL

Eligibility

Min Age

3 Months

Max Age

5 Years

Sex

MALE

Healthy Volunteers

No

Timeline & Regulatory

Start

2022-12-19

Primary Completion

2023-05-31

Completion

2023-08-31

Countries

• Pakistan

Study Locations