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Heterogeneity of Dendritic Cells in Colon and Non-small Cell Lung Cancer

NCT04789252 · Status: RECRUITING · Type: OBSERVATIONAL · Enrollment: 64

Last updated 2024-01-02

No results posted yet for this study

Summary

Prospective study with the use of biological samples. The centers involved are the Thoracic Surgery and Surgery 1 Units of the San Gerardo Hospital in Monza.

Conditions

Interventions

BIOLOGICAL

Bioinformatics analysis of single cell transcriptomics data

1. Sample collection: Myeloid cell purification + Single cell sequencing 2. Primary analysis: QC sequencing data + Reads alignment + UMI quantification 3. Bioinformatics analysis: Quality Control (Number of genes, UMI content,%mitochondrial genes, % riboprotein genes,Filtering low quality cells) + Normalization and scaling (Normalization, Remove unwanted sources of variation, HVG detection) + PCA Clustering Markers (PCA and PC selection, Clustering, t-SNE, Differential expression for markers identification) 4. Validation: FACS analysis + immunohistochemistry

Sponsors & Collaborators

  • University of Milano Bicocca

    lead OTHER

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2021-02-09
Primary Completion
2026-02-09
Completion
2026-02-09

Countries

  • Italy

Study Locations

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04789252 on ClinicalTrials.gov