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Hypotaurine Supplementation Benefits in Cryopreservation

NCT04011813 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 33

Last updated 2019-07-09

No results posted yet for this study

Summary

Although it is widely used, slow freezing can induce strong functional and nuclear spermatic alterations reducing the chances of pregnancy. The study objective is to determinate the effects of the combination of hypotaurine supplementation and spermatozoa selection by Density Gradient Centrifugation (DGC) on human sperm functions and DNA quality during a freezing-thawing cycle.

Conditions

  • Cryopreservation
  • Human Spermatozoa Parameters
  • Hypotaurine Supplementation
  • Spermatozoa DNA Alterations

Interventions

OTHER

Hypotaurine : antioxidant and osmoregulator

Hypotaurine has protective effects on sperm motility, capacitation and acrosome reaction and reduces apoptotic markers. Hypotaurine (50mM) was added in density gradient centrifugation, washing and cryopreservation media washing and cryopreservation media before spermatozoa freezing

Sponsors & Collaborators

  • Université d'Auvergne, Institut National de la Santéet de la Recherche Médicale

    collaborator UNKNOWN

  • Université d'Auvergne, Centre National de la Recherche Scientifique

    collaborator UNKNOWN

  • University Hospital, Clermont-Ferrand, Hematology laboratory

    collaborator UNKNOWN

  • University Hospital, Clermont-Ferrand

    lead OTHER

Principal Investigators

  • Florence BRUGNON, MD, PhD · University Hospital, Clermont-Ferrand

Eligibility

Min Age
18 Years
Sex
MALE
Healthy Volunteers
No

Timeline & Regulatory

Start
2014-05-01
Primary Completion
2018-08-01
Completion
2019-05-01

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT04011813 on ClinicalTrials.gov