Effect of Sperm Selection Techniques on Human Neonatal Gender Ratio in Patients Undergoing ICSI

Summary

babies of known gender born out of 388 ICSI cycles were investigated for the gender ratio and then divided into three groups according to the sperm selection technique used before performing sperm injection. Statistical analysis were made to compare ratios and compare results of three arms

Conditions

• Gender Ratio of ICSI Outcome

Interventions

OTHER

Density Gradient Centrifugation

semen was layered over 50 % and 90% discontinuous Density Gradient layers in a 15ml conical tube, then centrifuged at 250 g for 8 min at room temperature. supernatant was aspirated and the resulted pellet was washed using Sperm wash media and centrifuged at 250 g for 8 min at room temperature. The final pellet was re suspended in residual volume

DEVICE

Physiological ICSI

Semen processing is done by double layer density gradient method followed by adding Sperm to the dot of hyaluronan on the PICSI dish, within minutes the bound sperm are attached by their acrosome to the surface of the dot. Hyaluronan bound sperms are selected for oocyte injection

DEVICE

Magnetic Activated Cell Sorting

Semen processing is done by double layer density gradient method. The resulted pellet is labeled with Annexin V microbeads followed by separation on MACS column, the eluted fraction contains non apoptotic sperms suitable for oocyte injection.

Sponsors & Collaborators

• The Cleveland Clinic

  collaborator OTHER

• University of the Western Cape

  collaborator OTHER

• Ganin Fertility Center

  lead OTHER

Principal Investigators

• khaled Elqusi, BSc · Ganin Fertility Center

• Eman Hassanen, Bsc · Ganin Fertility Center

• Hanaa Alkhader, ABB( ELD) · Ganin Fertility Center

• Hosam Zaki, MSc, FRCOG · Ganin Fertility Center

• Ralph Henkel, PhD · University of the Western Cape

• Ashok Agarwal, PhD · American Center of Reproductive Medicine, Cleveland Clinic

Eligibility

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2016-08-01

Primary Completion

2019-03-30

Completion

2019-03-30

Countries

• Egypt

Study Locations