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Candidate Gene Screening for Attention Deficit/Hyperactive Disorder (ADHD)

NCT03018574 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 100

Last updated 2018-02-26

No results posted yet for this study

Summary

Source: Sample bank of Xijing Hospital and Children's Hospital Affiliated to Soochow University; Sample form: Whole blood; Estimated number of samples: 100 patients with ADHD and age, sex matched healthy controls; Case exclusion criteria: all kinds of neuropsychiatric disorders, IQ value of less than 70;

Study protocol:

1. Using Qiagen kit to extract the genomic DNA of 200 microliters of blood.
2. UV spectrophotometer test DNA purity of 260/280 close to 1.8 (1.8 ± 0.05), the concentration of 100ng/μL or more before the next sequencing.
3. The extracted genomic DNA will be sent to Sangon Biology Engineering Limited Company (Shanghai) and sequenced to find candidate mutations related to ADHD risk sequence. According to NIH gene database, the longest transcript of NDRG2 (ID: 57447 gene, https://www.ncbi.nlm.nih.gov/nuccore/NC\_000014.9? Report=genbank\&from=21016763\&to=21070872\&strand=true) (a total of 17 exons and 16 introns and the gene 5 'UTR and 3' UTR region) will be alignmented sequences to find potential mutations.
4. Using the chi square analysis and other statistical methods to determine the relationship between the mutations and susceptibility to ADHD.

Conditions

  • Gene Product Sequence Variation

Sponsors & Collaborators

  • Soochow University

    collaborator OTHER

  • Xijing Hospital

    lead OTHER

Principal Investigators

  • Yan Li, PhD&MD · Xijing hospital of The fourth military medical university

Eligibility

Min Age
6 Years
Max Age
14 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2016-05-31
Primary Completion
2017-01-31
Completion
2017-01-31

Countries

  • China

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT03018574 on ClinicalTrials.gov