Anisakis Blastocystis Cryptosporidium Fish Serology

Summary

Anisakidae frequently infect fish species that are commonly eaten by humans. Some of them are recognized as zoonotic diseases agents, and have a high impact on human health.

Infestation results from the ingestion of living larvae from contaminated fishes. It can be asymptomatic or symptomatic, resulting in acute gastric, acute intestinal or chronic forms. Allergic manifestations are frequently encountered in gastric forms, but allergic symptoms can also occur in isolation, after ingestion of Anisakidae antigens contained in raw or cooked fish, and may masquerade as fish allergy.

In this study, we aim to characterize the relationship between Anisakidae and/or fish sensitization and the presence of allergic manifestations in patients recruited in the general population and presenting fish allergy resulting from ingestion, cutaneous or respiratory contact. We will also determine the respective role of Anisakidae or fish sensitization in patients with fish allergy. Then, we will determine the prevalence of previous Anisakidae infections among these patients and a matched control population. We will also compare the performances of serological tests (ImmunoCAP, immunoelectrophoresis and Western Blot) for the diagnosis of Anisakidae allergy or infection. Lastly, we will explore the relationship between domestic exposure to Anisakidae or fish antigens and the occurrence of associated pathologies (Anisakidae or fish allergy/sensitization; Anisakidae infection).

Conditions

• Allergy to Fish
• Allergy
• Anisakis

Interventions

OTHER

Questionnaire

Questionnaire on domestic exposure to fish, and, for patients with fish allergy, on the characteristics of clinical manifestations)

BIOLOGICAL

Detection of anti-Anisakis and anti-fish antibodies

Anti-fish IgE will be detected using ImmunoCAP. Anti-Anisakis antibodies will be detected using ImmunoCAP (IgE), immunoelectrophoresis (precipitins) and Western Blot.

Sponsors & Collaborators

• Institut Pasteur de Lille

  collaborator OTHER

• ANSES

  collaborator OTHER

• Hôpital Cochin

  collaborator OTHER

• Région Nord-Pas de Calais, France

  collaborator OTHER

• University Hospital, Lille

  lead OTHER

Principal Investigators

• Emmanuel DUTOIT, MD · University Hospital, Lille

Eligibility

Min Age

18 Years

Max Age

65 Years

Sex

ALL

Healthy Volunteers

Yes

Timeline & Regulatory

Start

2015-02-28

Primary Completion

2017-01-31

Completion

2017-01-31

Countries

• France

Study Locations