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A New Method for Detection of Bacteria in the Bloodstream

NCT02323165 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 14

Last updated 2022-07-07

No results posted yet for this study

Summary

The primary aim is to determine if this new technique will detect and identify bacteria in the blood sooner than standard blood cultures or identify patients who may be septic without growing bacteria in their cultures. These will be correlated with the data collected from medical records on presumed sepsis. These results will be linked to data concerning infection that will be available as part of routine care including blood counts and other laboratory values that would be part of the routine medical care such as a white blood cell count. The earlier the bacteria are identified and the appropriate antimicrobials are administered the better the patient outcome.

Conditions

  • Burns
  • Wounds

Interventions

OTHER

Molecular Detection of Bacteria in the Bloodstream

Extracted nucleic acid will be tested by Polymerase Chain Reaction (PCR) with universal bacterial 16 S amplifiers from known 16 S sequences that routinely contaminate reagents. Polymerase Chain Reaction (PCR) will be performed with both TaqMan assays to provide quantitative copy numbers, as well as traditional PCR that can produce products that can be sequenced to confirm bacterial species identification.

Sponsors & Collaborators

  • University of Florida

    lead OTHER

Principal Investigators

  • Brenda Fahy, MD · University of Florida

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2015-01-31
Primary Completion
2022-03-04
Completion
2022-03-04

Countries

  • United States

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT02323165 on ClinicalTrials.gov