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Day-3 vs Day-5 Assisted Hatching: Impact on Blastocyst Morphology and PGT-A Outcomes

NCT07154875 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 158

Last updated 2026-08-18

No results posted yet for this study

Summary

This randomized controlled trial compared assisted hatching (AH) performed on Day 3 versus Day 5 in patients undergoing IVF-ICSI with preimplantation genetic testing for aneuploidy (PGT-A). Eligible participants were randomly assigned to one of the two AH strategies, and all embryos from each IVF-ICSI cycle followed the assigned strategy. The study evaluated whether the timing of AH was associated with differences in blastocyst development, blastocyst morphology, and PGT-A outcomes. The findings are intended to help optimize the timing of assisted hatching in IVF laboratory practice.

Conditions

  • Infertility; In Vitro Fertilization; Embryo Development

Interventions

PROCEDURE

Laser-Assisted Hatching

A standardized laser-assisted opening of the zona pellucida was performed either on Day 3 or Day 5 according to randomized group assignment. The only protocol-defined difference between study arms was the timing of assisted hatching; subsequent blastocyst culture, Day-5 assessment, and trophectoderm biopsy procedures were standardized across both groups.

Sponsors & Collaborators

  • 16A Ha Dong General Hospital

    collaborator OTHER

  • Hoang Minh Ngan

    lead OTHER

Principal Investigators

  • Ngan M Hoang, MSc · Center for Reproductive Support, 16A Ha Dong General Hospital

  • Tao D Nguyen, MD, PhD, Professor · Center for Reproductive Support, 16A Ha Dong General Hospital

  • Ton D Nguyen, PhD · Institute of Biology, Vietnam Academy of Science and Technology

  • Son T Trinh, MD, PhD · Vietnam Military Medical University, Ministry of National Defence

Study Design

Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
TRIPLE
Model
PARALLEL

Eligibility

Min Age
18 Years
Sex
FEMALE
Healthy Volunteers
No

Timeline & Regulatory

Start
2025-06-14
Primary Completion
2026-07-07
Completion
2026-07-07

Countries

  • Vietnam

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07154875 on ClinicalTrials.gov