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Fertility Preservation in Children With Solid Tumors: Detection of Residual Disease by a Sensitive Method

NCT07141862 · Status: RECRUITING · Type: OBSERVATIONAL · Enrollment: 89

Last updated 2025-09-03

No results posted yet for this study

Summary

In prepubertal patients, cryopreservation of ovarian or testicular tissue is currently the only available method for fertility preservation prior to gonadotoxic cancer treatments. However, this approach carries the risk of reintroducing malignant cells upon autotransplantation, particularly in cases of metastatic cancers such as neuroblastoma and Ewing sarcoma. Therefore, it is crucial to employ highly sensitive techniques to detect minimal residual disease (MRD) in preserved gonadal tissues.

This study aims to identify the most effective detection method by comparing the sensitivity and specificity of reverse transcription quantitative PCR (RT-qPCR) and droplet digital PCR (ddPCR) in identifying MRD of neuroblastoma and Ewing sarcoma in ovarian and testicular tissues from patients treated for these malignancies during infancy.

Conditions

Interventions

DIAGNOSTIC_TEST

RT-qPCR

Detection of MRD of neuroblastoma and Ewing sarcoma in ovarian and testicular tissues using RT-qPCR

DIAGNOSTIC_TEST

ddPCR

Detection of MRD of neuroblastoma and Ewing sarcoma in ovarian and testicular tissues using ddPCR

Sponsors & Collaborators

  • University Hospital, Rouen

    collaborator OTHER

  • University Hospital, Clermont-Ferrand

    lead OTHER

Principal Investigators

  • Florence BRUGNON, MD, PhD, HDR · University Hospital, Clermont-Ferrand

Eligibility

Min Age
2 Years
Max Age
45 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2025-02-01
Primary Completion
2027-06-30
Completion
2027-12-31

Countries

  • France

Study Locations

More Related Trials

Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07141862 on ClinicalTrials.gov