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Effect of Acute Exercise on Biochemical Parameters in Normal-Weight and Overweight/Obese Adolescent.

NCT07103889 · Status: COMPLETED · Phase: NA · Type: INTERVENTIONAL · Enrollment: 35

Last updated 2025-09-11

No results posted yet for this study

Summary

The goal of this interventional clinical trial is to compare the acute physiological and biochemical responses to maximal physical exercise (Spartacus test). in two groups of adolescent girls: in normal-weight and overweight/obese adolescent. The main question it aims to answer:

* Does Spartacus test significantly alter glucose metabolism and lipid profile in normal-weight and overweight/obese adolescent?
* How do inflammatory markers such as C-Reactive Protein \[CRP\], Erythrocyte Sedimentation Rate \[ESR\], respond immediately after and 30 minutes following maximal effort?
* Are there significant changes in liver enzymes (Aspartate Aminotransferase \[AST\], Alanine Aminotransferase \[ALT\]), muscle damage indicators (creatine kinase \[CK\], Lactate Dehydrogenase \[LDH\]), and electrolyte balance post-exercise? Participants will undergo a baseline blood draw before performing the Spartacus test , a progressive maximal exercise test. Blood samples will then be collected immediately after the test and again 30 minutes post-exercise to evaluate changes in: Glucose metabolism (fasting glucose), Lipid profile (total cholesterol, High-Density Lipoprotein \[HDL\], Low-Density Lipoprotein \[LDL\], triglycerides), Liver and muscle enzymes (Aspartate Aminotransferase \[AST\], Alanine Aminotransferase \[ALT\], Creatine kinase \[CK\], Lactate Dehydrogenase \[LDH\]), Renal function markers (creatinine, urea), Inflammatory markers (C-Reactive Protein \[CRP\], Erythrocyte Sedimentation Rate \[ESR\]), Hematological profile (complete blood count: White Blood Cells \[WBC\], Red Blood Cells \[RBC\], Hemoglobin \[Hb\], Hematocrit \[Hct\], platelets), Electrolytes (sodium, potassium, chloride), Uric acid, calcium, phosphorus, magnesium, iron.

Conditions

Interventions

BEHAVIORAL

Spartacus Test

The Spartacus test was conducted on a 750 m (75 × 10 m) rectangular course marked with cones at regular intervals to indicate different speeds (from 7 to 18 km/h). Each participant began running at 7 km/h, with speed increasing by 1 km/h every three minutes.During the 3-minute intervals (for each speed stage), participants had 15 seconds to reach the corresponding marker, followed by 15 seconds of rest. Participants had to adjust their running pace to reach the target cone in synchrony with an audio signal. The test ended when a participant failed to reach the next cone within the given time. This test is validated for assessing maximal aerobic capacity (MAS) in obese adolescents. During the Spartacus test, maximal heart rate (HRmax) was recorded using a heart rate monitor (S810; Polar, Kempele, Finland).

Sponsors & Collaborators

  • High Institute of Sports and Physical Education of Kef

    lead OTHER

Principal Investigators

  • Wissal abassi, Dr · Research Unit "Sport Sciences, Health and Movement"(UR22JS01) High Institute of Sport and Physical Education of Kef, University of Jendouba, 7100 Kef, Tunisia.

Study Design

Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Model
PARALLEL

Eligibility

Min Age
13 Years
Max Age
18 Years
Sex
FEMALE
Healthy Volunteers
No

Timeline & Regulatory

Start
2025-08-10
Primary Completion
2025-08-13
Completion
2025-08-13

Countries

  • Tunisia

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Entities

Diseases

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT07103889 on ClinicalTrials.gov