Trial Outcomes & Findings for Mechanisms and Phenotypes of Hypertension in Patients in Chronic Hemodialysis (NCT NCT06764277)

NCT ID: NCT06764277

Last Updated: 2026-02-03

Results Overview

Cardiac output (systolic volume x heart rate) was determined by 2 experienced observers using a Siemens Acuson P500 ultrasound equipment with a phased array transducer . Systolic volume was calculated with the velocity time integral of the flow of the left ventricle outflow tract of the aortic area.

Recruitment status

COMPLETED

Target enrollment

32 participants

Primary outcome timeframe

Each participant was studied once 1-3 hours after dialysis ended

Results posted on

2026-02-03

Participant Flow

Participant milestones

Participant milestones
Measure
Group A (ABP < 130/80 mmHg)
Constituted by15 patients in the chronic hemodialisis program with ABP \< 130/80 mmHg, and we measured interdialytic weight gain, cardiac output, systemic vascular resistance, blood pressure changes, interdialytic ambulatory blood pressure monitoring (ABPM) . Serum levels of angiotensin II, norepinephrin, copeptin and the ratio of monocytes + neutrophils / linphocytes (MNLR)
Group B (ABP 130-139/80-89 mmHg)
This group was constituted by patients with ABP 130-139/80-89 mmHg. They were 7 patients in the chronic hemodialisis program, and we measured blood pressure changes, interdialysis ambulatory blood pressure monitoring (ABPM) and interdialisis weight gain. Serum levels of angiotensin II, norepinephrin, copeptin and the ratio of monocytes + neutrophils / lymphocytes (MNLR).
Group C (ABP ≥ 140/90 mmHg)
Constituted by 10 patients in the chronic hemodialisis program, with ABP ≥ 140/90 mmHg. We measured blood pressure changes, interdialysis ambulatory blood pressure monitoring (ABPM) and interdialisis weight gain. Serum levels of angiotensin II, norepinephrin, copeptin, and the ratio of monocytes + neutrophils / linphocytes (MNLR)
Overall Study
STARTED
15
7
10
Overall Study
COMPLETED
15
7
10
Overall Study
NOT COMPLETED
0
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Group A (ABP < 130/80 mmHg)
n=15 Participants
We observed 15 patients in the chronic hemodialysis program with ABP \< 130/80 mmHg, and we measured blood pressure changes, interdialytic ambulatory blood pressure monitoring and interdialytic weight gain. Serum levels of angiotensin II, norepinephrine, copeptin, and the ratio of monocytes + neutrophils / lymphocytes (MNLR).
Group B (ABP 130-139/80-89 mmHg)
n=7 Participants
We observed 7 patients in the chronic hemodialysis program with ABP 130-139/80-89 mmHg , and we measured blood pressure changes, interdialytic ambulatory blood pressure monitoring and interdialysis weight gain. Serum levels of angiotensin II, norepinephrine, copeptin, and the ratio of monocytes + neutrophils / lymphocytes (MNLR).
Group C (ABP ≥ 140/90 mmHg)
n=10 Participants
We observed 10 patients in the chronic hemodialysis program with ABP ≥ 140/90 mmHg and we measured blood pressure changes, interdialytic ambulatory blood pressure monitoring and interdialytic weight gain. Serum levels of angiotensin II, norepinephrine, copeptin, and the ratio of monocytes + neutrophils / lymphocytes (MNLR).
Total
n=32 Participants
Total of all reporting groups
Age, Continuous
38 years
n=15 Participants
44 years
n=7 Participants
35 years
n=10 Participants
39 years
n=32 Participants
Sex: Female, Male
Female
12 Participants
n=15 Participants
2 Participants
n=7 Participants
5 Participants
n=10 Participants
19 Participants
n=32 Participants
Sex: Female, Male
Male
3 Participants
n=15 Participants
5 Participants
n=7 Participants
5 Participants
n=10 Participants
13 Participants
n=32 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
Mexico
15 participants
n=15 Participants
7 participants
n=7 Participants
10 participants
n=10 Participants
32 participants
n=32 Participants
Cardiac output
3.88 L/min
STANDARD_DEVIATION 0.99 • n=15 Participants
4.10 L/min
STANDARD_DEVIATION 0.77 • n=7 Participants
4.55 L/min
STANDARD_DEVIATION 1.21 • n=10 Participants
4.25 L/min
STANDARD_DEVIATION 0.34 • n=32 Participants
Hypertension Phenotypes
Dippers
5 Participants
n=15 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
3 Participants
n=7 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
1 Participants
n=10 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
9 Participants
n=32 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
Hypertension Phenotypes
Non-dippers
5 Participants
n=15 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
2 Participants
n=7 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
4 Participants
n=10 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
11 Participants
n=32 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
Hypertension Phenotypes
Extreme Dippers
1 Participants
n=15 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
0 Participants
n=7 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
0 Participants
n=10 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
1 Participants
n=32 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
Hypertension Phenotypes
Reverse-dippers
4 Participants
n=15 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
2 Participants
n=7 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
5 Participants
n=10 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32
11 Participants
n=32 Participants • Number of participants indicate the participants analized in groups A, B and C (all members of each group (total of 32 participantes). The number of deepers, non-deepers, extreme deepers and reverse deepers was different in each group but the total number is also 32

PRIMARY outcome

Timeframe: Each participant was studied once 1-3 hours after dialysis ended

Population: Patients were mexicans of both sexes of mixed (undetermined) race and ethnicity

Cardiac output (systolic volume x heart rate) was determined by 2 experienced observers using a Siemens Acuson P500 ultrasound equipment with a phased array transducer . Systolic volume was calculated with the velocity time integral of the flow of the left ventricle outflow tract of the aortic area.

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Cardiac Output
4.6 L/min
Standard Error 0.38
3.9 L/min
Standard Error 0.26
4.10 L/min
Standard Error 0.29

PRIMARY outcome

Timeframe: Interdialytic weight change was studied once, by the difference between the weight at the end of dialysis and the weight 2-3 days later, before the next dialysis.

Population: Patients were mexicans of both sexes and mixed (undetermined) race and ethnicity

The weight was determined in 2 ocassions: first, immediately after dialysis and second, 2-3 days later, before the next dialysis. The weight change in this interval is expressed as percentage change of the second measure in relation to the first measure.

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Interdialytic Weight Change
2.98 percentage change
Standard Error 0.53
2.90 percentage change
Standard Error 0.37
3.22 percentage change
Standard Error 0.63

PRIMARY outcome

Timeframe: Systemic vascular resistance was determined once in the participants 1-3 hours after dialysis

Population: All the participants were mexicans of both sexes and mixed (undetermined) race and ethnicity

Systemic vascular resistance was calculated using MediCalcR using the mean arterial pressure (MAP), the central venous pressure (CVP) anf the cardiac output (CO) and the equation: SVR=\[(MAP-CVP)x79.92\]/CO. Cardiac output (CO= systolic volume x heart rate) and central venous pressure were determined by 2 experienced observers using a Siemens Acuson P500 ultrasound equipment with a phased array transducer. Systolic volume was calculated with the velocity time integral of the flow of the left ventricle outflow tract of the aortic area.

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Systemic Vascular Resistance
1751 dynes*s/cm˄5
Standard Error 187
1513 dynes*s/cm˄5
Standard Error 114.6
2045 dynes*s/cm˄5
Standard Error 161.0

SECONDARY outcome

Timeframe: Each participant was studied once. Serum samples taken before dialysis

Population: The participants were mexican of both sexes and mixed (undetermined) race and ethnicity

Angiotensin serum levels (pg/ml) were determined once at the end of the interdialytic period, before dialysis, using ELISA (my BioSource Cat.# MBS703599)

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Angiotensin II Serum Levels
231.5 pg/ml
Standard Error 40.4
105.3 pg/ml
Standard Error 12.9
158.3 pg/ml
Standard Error 43.8

SECONDARY outcome

Timeframe: Each participant was studied once. Blood samples taken before dialysis (following the end of the interdialytic period).

Population: Participants were mexicans of mixed (undetermined) race

Monocyte+Neutrophile/lymphocyte ratio obtained from peripheral blood counts in samples taken before dialysis (following the end of the interdialytic period).

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Monocyte+Neutrophiles/Lymphocyte Ratio (MNLR)
1.11 ratio
Standard Error 0.13
0.88 ratio
Standard Error 0.13
0.73 ratio
Standard Error 0.09

SECONDARY outcome

Timeframe: Each participant was studied once. Serum samples taken before dialysis

Population: The studies were done at the end of the interdialytic period. Samples taken before the next dialysis

Interdialytic serum norepinephrine levels determined in blood samples obtained before dialysis (end of interdialytic period) using ELISA (ABCAM, Cat. # AB287789);

Outcome measures

Outcome measures
Measure
Group C
n=10 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=15 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=7 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Serum Norepinephrine Levels
82.6 pg/ml
Interval 74.9 to 259.0
81.4 pg/ml
Interval 68.5 to 87.4
82.4 pg/ml
Interval 66.5 to 165.4

SECONDARY outcome

Timeframe: Each participant was studied once. Serum samples taken before dialysis

Population: Mexican mixed population of undetermined race

The copeptin levels (surrogate for arginine vasopressin) were measured before dialyisis (end of the interdialytic period) using ELISA (Bio-techne/NovusBiological, Cat, # NBP2-69822)

Outcome measures

Outcome measures
Measure
Group C
n=9 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=11 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=6 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
Nocturnal blood pressure higher than diurnal blood pressure
Serum Copeptin Levels
13.0 pmol/L
Standard Error 1.56
14.6 pmol/L
Standard Error 1.09
10.3 pmol/L
Standard Error 0.92

SECONDARY outcome

Timeframe: Each participant was studied once. Ambulatory blood pressure was monitored or 24-44 hours during the interdialytic perdiod starting 1-3 hours after dialysis

Population: The number of dippers, non-dippers, extreme dippers and reverse dippers were studied in all participants (n=32) .

Interdialytic ambulatory blood pressure determined during 24-44 hours using Space Labs Health Care, model 90217A and Contec ABPM50 equipment. Determinations every 30 minutes during daytime and every hour during the night. We are here reporting the phenotypes in the total number of participants (n=32) instead that in each group (groups A n=15, B n=7 and C n=10) because our main interest was to determine the incidence of each phenotyte in stable patients treated with chronic dialysis, rather than in each group that, individually, had few patients

Outcome measures

Outcome measures
Measure
Group C
n=32 Participants
onstituted by 10 patients with ≥140/90 mmHg
Group A
n=32 Participants
Constituted by 15 patients with ABP \<130/80.
Group B
n=32 Participants
Constituted by 7 patients with ABP 130-139 mmHg.
Reverse Dipper
n=32 Participants
Nocturnal blood pressure higher than diurnal blood pressure
Hypertension Phenotypes
13 Participants
8 Participants
1 Participants
10 Participants

Adverse Events

Group 1 (TA < 130mmHg)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Group 2 (TA 130-139mmHg)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Group 3 (TA > 140)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Bernardo Rodriguez Iturbe

Instituto Nacional de Ciencias Medicas y Nutricion "Salvador Zubiran"

Phone: 9991490061

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place