Adversity and Its Association With the Development and Expression of Rheumatic Diseases

Summary

Epidemiological evidence shows that adverse experiences, particularly, but not exclusively in childhood, are predictors of poor long-term health outcomes and certain social domains. In the field of rheumatic diseases, traumatic events, not only in childhood, have been associated with hospitalization, chronic pain, inflammation, worse outcomes, severity of the disease, and mortality. Some mechanisms proposed to explain the association between the experience of adversity and the development of chronic diseases include an impact on the physiology of immune system cells, gene expression due to DNA modification, and cellular senescence.

With this background, the investigators wonder if, for patients with rheumatoid arthritis, the presence of adversity understood as a history of violence in childhood and abuse due to suffering from rheumatoid arthritis is associated with markers of cellular senescence and with the severity of illness.

Conditions

• RhA - Rheumatoid Arthritis

Interventions

OTHER

Rheumatic diseases Mistreatment Scale (RDMS)

In 2012, Giraldo-Rodríguez developed and validated the Geriatric Mistreatment Scale (GAS); translation, cultural adaptation, and validation were performed prior to its application.

OTHER

Routine assessment of patient index data 3 (RAPID-3)

RAPID- 3 measures: function, pain, and patient global status estimate. Each of the three individual measures is scored 0 to 10, for a total of 30

OTHER

Health Assessment Questionnaire (HAQ)

The HAQ is based on five patient-centered dimensions: disability, pain, medication effects, costs of care, and mortality.

OTHER

WHOQOL-BREF

WHOQOL-BREF is a 26-item instrument consisting of four domains: physical health, psychological health, social relationships, and environmental health; it also contains QOL and general health items

OTHER

Depression, Anxiety and Stress Scale (DASS-21)

DASS-21 is a set of three self-report scales designed to measure the emotional states of depression, anxiety, and stress. Each of the three DASS-21 scales contains seven items, divided into subscales with similar content.

OTHER

Brief Resilient Coping Scale

The Brief Resilient Coping Scale is a 4-item measure designed to capture tendencies to cope with stress in a highly adaptive manner.

GENETIC

Expression of CDKN2A /p16INK4a

CDKN2A/p16INK4a expression will be measured using the Taqman qPCR assay (TaqMan Universal Master Mix II, with UNG, Applied Biosystems, Foster City, USA) according to the manufacturer's specifications.

OTHER

Immunophenotype of leukocyte subpopulations

CD4+ and CD8+ subpopulations will be analyzed. Blood samples will be analyzed by 8-color flow cytometry (Becton Dickinson Canto II cytometer) using fluorescently labeled antibodies from Biolegend Inc. (San Diego, USA).

GENETIC

Telomere length

The rLTL will be estimated by quantitative monochromatic multiplex PCR (MM-qPCR). Integrated DNA Technologies, Inc. (IDT, Coralville, IA, USA) will synthesize the primers used for telomere measurements.

OTHER

Cellular senescence

Expression of co-stimulatory molecules and surface receptors CD27- and CD28-

Sponsors & Collaborators

• National Institute of Medical Sciences and Nutrition, Salvador Zubiran

  lead OTHER

Principal Investigators

• Virginia MD Pascual-Ramos, MD · IInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Eligibility

Min Age

18 Years

Max Age

100 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2024-06-01

Primary Completion

2026-01-01

Completion

2026-02-01