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MicroRNA-1 (miRNA-1) and MicroRNA-133a (miRNA-133a) Levels After Acute Exercise in Ultimate Frisbee Athletes

NCT06083610 · Status: RECRUITING · Type: OBSERVATIONAL · Enrollment: 34

Last updated 2023-10-16

No results posted yet for this study

Summary

"The goal of this clinical trial is to investigate the levels of microRNA-1 (miRNA-1) and microRNA-133a (miRNA-133a) after acute neuromuscular exercise (NME) in human saliva.

The main questions it aims to answer are:

* How do miRNA-1 and miRNA-133a levels change in response to acute neuromuscular exercise in human saliva?
* Will the chosen training method have an acute effect in ultimate frisbee athletes?

Participants will:

* Undergo acute neuromuscular exercise (NME).
* Provide saliva samples for miRNA analysis.

Researchers will compare:

MiRNA-1 and miRNA-133a levels before and after acute neuromuscular exercise (NME) to determine if exercise induces any significant changes in these microRNA levels."

Conditions

  • To Determine the Levels of miRNA1 and miRNA133a Before and After Exercise

Interventions

OTHER

miRNA levels analysis from saliva samples

Each participant will be given two 50-mL Falcon tubes and will be asked to expectorate a small amount (about 1 mL) of saliva before and immediately after the end of exercise. Part of each saliva sample will be mixed with an equal volume of denaturing solution included in the mirVanaTM PARISTM kit from Ambion, Foster City, CA, in order to inactivate ribonucleases. Serum samples will be separated by centrifugation at 4000 rpm from the saliva samples taken into the biochemistry tubes of the participants and controls and will be raised to -80 centigrade degrees until the study day. Micro RNA will be isolated from serum samples on the working day. Determination of microRNA expression levels (micro RNA-1 and micro RNA-133a) will be determined by reverse transcriptase polymerase chain reaction (RT-PCR) (Applied Biosystems 7500 Fast Real-Time PCR).

Sponsors & Collaborators

  • Yeditepe University

    lead OTHER

Principal Investigators

  • Turgay İsbir, Prof. Dr. · Yeditepe University

Eligibility

Min Age
18 Years
Max Age
25 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2023-10-11
Primary Completion
2024-04-30
Completion
2024-05-31

Countries

  • Turkey (Türkiye)

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT06083610 on ClinicalTrials.gov