Metabo-lipidomics of the Ocular Surface for Cataract Surgery

Summary

Cataract is the world's leading cause of blindness, the treatment of which, exclusively surgical, offers excellent results in terms of visual recovery. It represents the most frequently performed surgery in France with more than 800,000 procedures each year. However, some patients develop postoperative ocular surface disease that can affect final visual acuity and quality of life. For example, dry eye disease, very common in the elderly, can be worsened by cataract surgery.

The identification of ocular surface biomarkers predictive of the postoperative risk of ocular surface disease carries the promise of better personalized perioperative care.

Conjunctival impression cytology represents a rapid, minimally invasive method of collecting conjunctival cells, which has proven its usefulness in the evaluation of diseases of the ocular surface. The aqueous humor is directly accessible at the beginning of the surgery.

An approach combining ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry on the cells of the ocular surface and on the aqueous humor could improve our understanding of the physiopathology of ocular surface disease following cataract surgery.

This study will aim to 1) search for prognostic biomarkers of ocular surface disease after cataract surgery using a metabo-lipidomic approach 2) improve our understanding of the pathophysiological mechanisms involved.

Conditions

• Age-related Cataract

Interventions

OTHER

Oxford score

Investigation and quantification of superficial corneal punctate keratitis using the Oxford score

OTHER

Conjunctival collection

Two conjunctival prints: one for metabolomic and one for lipidomic

OTHER

Ocular Surface Disease Index

Questionnaire on ocular surface disorders

OTHER

Interferometry

Non-contact examination measuring NIBUT (non-invasive break-up time), meibography and tear meniscus measurement

OTHER

Aqueous humor collection

Collection of 0.1 mL of aqueous humor immediately after making the accessory corneal incision at the beginning of the cataract surgery.

OTHER

Quantification of Tyndall and flare

Quantification of Tyndall and flare (from 1 to 4 crosses) in anterior chamber

Sponsors & Collaborators

• University Hospital, Tours

  lead OTHER

Study Design

Allocation

NA

Purpose

DIAGNOSTIC

Masking

NONE

Model

SINGLE_GROUP

Eligibility

Min Age

50 Years

Sex

ALL

Healthy Volunteers

No

Timeline & Regulatory

Start

2023-09-04

Primary Completion

2025-07-09

Completion

2025-07-09

Countries

• France

Study Locations