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Molecular Detection Of Efflux Pump and Virulence Factors Genes in Pseudomonas Aeruginosa

NCT05642767 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 75

Last updated 2023-04-12

No results posted yet for this study

Summary

Pseudomonas aeruginosa (PA) is a ubiquitous aerobic, non-fermentative Gram-negative rod that is widely associated with nosocomial pneumonia and can lead to severe illness with poor outcomes, particularly in critically ill people due to the ability of some strains to cause lung epithelial injury and spread into the circulation. 2 In the intensive care unit, PA infection is ranked among the top five causes of the bloodstream, pulmonary, surgical site, urinary tract, and soft tissue infections.

Conditions

  • Pseudomonas Aeruginosa

Interventions

DIAGNOSTIC_TEST

culture on cetrimide agar

Samples will be inoculated on cetrimide agar using the plating out technique.

DIAGNOSTIC_TEST

Staining with Gram stain

colonies on cetrimide agar will be spread on glass slide and stained by gram stain

DIAGNOSTIC_TEST

Antibiotic sensitivity testing

Antibiotic sensitivity testing will be done by disc diffusion method according to CLSI

DIAGNOSTIC_TEST

Molecular detection to efflux genes and some virulence genes

Molecular detection to efflux genes and some virulence genes by conventional PCR

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Principal Investigators

  • Mohamed H Alrawy · Faculty of medicine, Sohag university

  • Ebtisam M Gad · Faculty Of Medicine, Sohag university

Eligibility

Min Age
4 Weeks
Max Age
80 Years
Sex
ALL
Healthy Volunteers
No

Timeline & Regulatory

Start
2022-12-01
Primary Completion
2023-03-30
Completion
2023-05-01

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05642767 on ClinicalTrials.gov