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Epithelial Mesenchymal Transition and Periodontitis

NCT05403164 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 80

Last updated 2022-06-03

No results posted yet for this study

Summary

Periodontitis is a chronic inflammatory disease results is destruction of the attachment apparatus of the teeth and ultimately tooth loss.

Epithelial-mesenchymal transition (EMT) is a process comprises of series of events that influence a polarized epithelial cell to undergo molecular/morphological changes leading to acquisition of mesenchymal cell phenotype. This process is responsible for suppressing epithelial-phenotype and it is known to be triggered by chronic exposure to inflammatory cytokines, Gram-negative bacteria, hypoxia, smoking, and hyperglycemia.

Both periodontitis and EMT share common risk factors/promoters; however, the role of EMT in the pathogenesis of periodontitis is not fully elucidated yet. Potential induction of EMT within periodontal pockets may disrupt epithelial barrier thus facilitating invasion of pathogenic periodontal pathogens to deeper tissues resulting in further tissue breakdown and non-resolving periodontal lesion.

Conditions

  • Periodontitis
  • Periodontal Diseases
  • Epithelial-Mesenchymal Transition
  • Transcription Factors
  • Epithelial Attachment
  • Gram-Negative Bacteria

Interventions

PROCEDURE

Modified Widman flap

1. Administration of anaesthesia 2. First incision (reversed bevel incision), scalpel is placed at 45 degree, 2mm apical to gingival margin in coronal-apical direction until touching the bone and moved continuously around the teeth without any vertical releasing incisions 3. Partial mobilization of the mucoperiosteal flap (full thickness flaps both facially and orally) within the attached gingiva to the alveolar crest 4. Second incision (sulcular incision) 5. Third incision (horizontal incision), also interdentally to remove the delineated tissue and all granulation tissue which is used later for analysis. 6. Root surface debridement 7. Flap adaptation, complete coverage interdentally and suturing.

PROCEDURE

Gingivectomy

1. Administration of anaesthesia 2. Marking the base of the sulcus with pocket marker tweezer. 3. First incision (gingivectomy incision), scalpel blade placed 1mm apical to the bleeding points and the incision should be beveled 45 degrees coronally. 4. Second incision (interdental) to free the tissue which is used for analysis later. 5. Debriding the area and applying periodontal pack.

Sponsors & Collaborators

  • University of Baghdad

    lead OTHER

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-07-12
Primary Completion
2022-08-30
Completion
2022-08-30

Countries

  • Iraq

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05403164 on ClinicalTrials.gov