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Plasma microRNA Levels and Some Cytokines Expression in Patients With ITP Primary Immune Thrombocytopenic Purpura (ITP)

NCT05371743 · Status: UNKNOWN · Type: OBSERVATIONAL · Enrollment: 2

Last updated 2023-04-12

No results posted yet for this study

Summary

Immune thrombocytopenia (ITP) is an autoimmune disease characterized by low platelet counts with or without mucocutaneous bleeding (McMillan, 2007). Like the majority of autoimmune diseases, ITP is an organ-specific disease, and abnormalities in the regulation of the immune system have been shown to play an important role in the initiation and/or perpetuation of the disease (McKenzie et al.,2013).

Still, immune thrombocytopenia (ITP) is a significant clinical problem due to chronicity, treatment cost, occurrence mainly in, young, and relatively poorer quality of life

Conditions

  • Primary Immune Thrombocytopenia

Interventions

DIAGNOSTIC_TEST

Plasma RNA isolation, qPCR analysis of micro RNA

qPCR will be performed using Taqman microRNA assays (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's protocol. Total RNAs going to be used to make cDNAs using TaqMan microRNA RT Kit. Diluted cDNAs were mixed with TaqMan Universal PCR Master Mix (No AmpErase UNG). Taqman miRNA assay will run in the 7500 Real-Time PCR System (Applied Biosystems). miR-39 also will be used as an exogenous control. All assays will be done in triplicates. The expression levels will be evaluated using the comparative cycle threshold (∆∆\_Ct) method

DIAGNOSTIC_TEST

estimation of serum level of IL2

2ml of patient serum will be used to measure IL 2 in patients with different groups by ELISA technique. The techniques will be done in the central research laboratory in Sohag university hospital

DIAGNOSTIC_TEST

estimation of serum level of IL17

2ml of patient serum will be used to measure IL17 in patients with different groups by ELISA technique. The techniques will be done in the central research laboratory in Sohag university hospital

Sponsors & Collaborators

  • Sohag University

    lead OTHER

Principal Investigators

  • Hend M Moness, professor · Faculty Of Medicine, Minya university

  • Aliaa S Abd EL Fatah, professor · Faculty Of Medicine, Minya university

  • Rasha F Ahmed, professor · Faculty of medicine, Minya university

Eligibility

Min Age
18 Years
Max Age
90 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2022-05-01
Primary Completion
2022-09-01
Completion
2023-09-01

Countries

  • Egypt

Study Locations

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Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05371743 on ClinicalTrials.gov