MedTrace Logo

Thyroid Gland Dysfunction and Vitamin D Polymorphism in Keratoconus

NCT05073601 · Status: COMPLETED · Type: OBSERVATIONAL · Enrollment: 262

Last updated 2021-10-21

No results posted yet for this study

Summary

This prospective study the thyroid gland dysfunction and vitamin D receptor polymorphism in keratoconus patients

Conditions

  • Keratoconus

Interventions

DIAGNOSTIC_TEST

ELISA,DNA extraction

quantitative measurement of (Thyroid stimulating hormon (TSH), free triiodothyronine (FT3)\& Free tetraiodothyronine(FT4)) measured using ELISA . Serum 25-OH vitamin D measured using ELISA. Serum 25-OH vitamin D will be classified based on 2012 American Endocrine Society guidelines into deficient (\< 20ng/ml), insufficient ( 21 -30 ng/ml) and sufficient (\> 30 ng/ml).vitamin D receptor polymorphisms was done in 3 steps: a) DNA extraction from whole blood using Gene Jet gene DNA Purification kits . b) PCR: three Vitamin D receptor polymorphisms were tested \[Taq I (restriction enzymes according to single nucleotide polymorphism ) (rs731236), Apa I (rs7975232) and Bsm I (rs1544410)\] using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was amplified using certain primers

Sponsors & Collaborators

  • Mansoura University

    lead OTHER

Principal Investigators

  • Eman Azmy · Mansoura University

Eligibility

Min Age
18 Years
Sex
ALL
Healthy Volunteers
Yes

Timeline & Regulatory

Start
2021-03-06
Primary Completion
2021-07-01
Completion
2021-09-01

Countries

  • Egypt

Study Locations

More Related Trials

Read the full study record

This page highlights key information. For complete eligibility criteria, study locations, investigator contacts, and the full protocol, visit the original record on ClinicalTrials.gov.

View NCT05073601 on ClinicalTrials.gov